Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28307 | 3' | -58.2 | NC_005905.1 | + | 19513 | 0.7 | 0.410939 |
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Target: 5'- aGGCCGGCUUGGaCCAAgCuAGCCUgGa -3' miRNA: 3'- -CUGGCCGAACUcGGUUgGcUCGGAgC- -5' |
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| 28307 | 3' | -58.2 | NC_005905.1 | + | 17859 | 0.67 | 0.587236 |
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Target: 5'- aGCuCGGCUUGuGCCGAgUuAGCUUCGa -3' miRNA: 3'- cUG-GCCGAACuCGGUUgGcUCGGAGC- -5' |
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| 28307 | 3' | -58.2 | NC_005905.1 | + | 17311 | 0.66 | 0.671098 |
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Target: 5'- aGCUaGCUUGgcccaAGCCAACCuGGUCUCGa -3' miRNA: 3'- cUGGcCGAAC-----UCGGUUGGcUCGGAGC- -5' |
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| 28307 | 3' | -58.2 | NC_005905.1 | + | 17421 | 0.66 | 0.671098 |
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Target: 5'- aGCUaGCUUGgcccaAGCCAACCuGGUCUCGa -3' miRNA: 3'- cUGGcCGAAC-----UCGGUUGGcUCGGAGC- -5' |
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| 28307 | 3' | -58.2 | NC_005905.1 | + | 17201 | 0.66 | 0.671098 |
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Target: 5'- aGCUaGCUUGgcccaAGCCAACCuGGUCUCGa -3' miRNA: 3'- cUGGcCGAAC-----UCGGUUGGcUCGGAGC- -5' |
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| 28307 | 3' | -58.2 | NC_005905.1 | + | 17091 | 0.66 | 0.671098 |
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Target: 5'- aGCUaGCUUGgcccaAGCCAACCuGGUCUCGa -3' miRNA: 3'- cUGGcCGAAC-----UCGGUUGGcUCGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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