Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28311 | 3' | -55.9 | NC_005905.1 | + | 49221 | 0.78 | 0.2028 |
Target: 5'- aGACCGGCUUGAGCCaACCGaGccUCGa -3' miRNA: 3'- -CUGGCCGAACUCGGaUGGCcCaaAGC- -5' |
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28311 | 3' | -55.9 | NC_005905.1 | + | 49176 | 0.78 | 0.2028 |
Target: 5'- aGACCGGCUUGAGCCgACCGaGccUCGa -3' miRNA: 3'- -CUGGCCGAACUCGGaUGGCcCaaAGC- -5' |
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28311 | 3' | -55.9 | NC_005905.1 | + | 49131 | 0.78 | 0.2028 |
Target: 5'- aGACCGGCUUGAGCCaACCGaGccUCGa -3' miRNA: 3'- -CUGGCCGAACUCGGaUGGCcCaaAGC- -5' |
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28311 | 3' | -55.9 | NC_005905.1 | + | 49086 | 0.78 | 0.2028 |
Target: 5'- aGACCGGCUUGAGCCaACCGaGccUCGa -3' miRNA: 3'- -CUGGCCGAACUCGGaUGGCcCaaAGC- -5' |
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28311 | 3' | -55.9 | NC_005905.1 | + | 49041 | 0.81 | 0.122676 |
Target: 5'- aGACCGGCUUGAGCCgACCGaGcUUCGg -3' miRNA: 3'- -CUGGCCGAACUCGGaUGGCcCaAAGC- -5' |
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28311 | 3' | -55.9 | NC_005905.1 | + | 49490 | 1.1 | 0.001399 |
Target: 5'- aGACCGGCUUGAGCCUACCGGGUUUCGg -3' miRNA: 3'- -CUGGCCGAACUCGGAUGGCCCAAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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