Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28316 | 3' | -54.2 | NC_005905.1 | + | 8815 | 0.67 | 0.797721 |
Target: 5'- uAGCUUcGAcaaauUUUaCCGAGUCCAGGCCg -3' miRNA: 3'- uUCGGA-CUc----AAAcGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 22087 | 0.67 | 0.788078 |
Target: 5'- uAGCUUcgacGAGUUUuaCCGAGUUUAGGCCg -3' miRNA: 3'- uUCGGA----CUCAAAc-GGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 20058 | 0.68 | 0.772324 |
Target: 5'- cGGCUUGGGccaagcuaGCuucgacaaauuuuauCGAGUCCAGACCg -3' miRNA: 3'- uUCGGACUCaaa-----CG---------------GCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 19883 | 0.68 | 0.772324 |
Target: 5'- cGGCUUGGGccaagcuaGCuucgacaaauuuuauCGAGUCCAGACCg -3' miRNA: 3'- uUCGGACUCaaa-----CG---------------GCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 22152 | 0.68 | 0.752128 |
Target: 5'- gAGGCCUGcuuuggccaAGUUaGCuucgacaaauuuuauCGAGUCUAGGCCg -3' miRNA: 3'- -UUCGGAC---------UCAAaCG---------------GCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 19773 | 0.68 | 0.741846 |
Target: 5'- cGGCUUGGGccaagcuaGCCucgacaaauuuuaucGAGUCCAGGCCg -3' miRNA: 3'- uUCGGACUCaaa-----CGG---------------CUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 8915 | 0.7 | 0.656612 |
Target: 5'- uGGGCa-GAGUUaGCuucgacaaauuuuacCGAGUCCAGGCCg -3' miRNA: 3'- -UUCGgaCUCAAaCG---------------GCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 21907 | 0.72 | 0.502528 |
Target: 5'- uAGCUUcgacGAGUUUuaCCGAGUCUAGGCCg -3' miRNA: 3'- uUCGGA----CUCAAAc-GGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 21862 | 0.72 | 0.502528 |
Target: 5'- uAGCUUcgacGAGUUUuaCCGAGUCUAGGCCg -3' miRNA: 3'- uUCGGA----CUCAAAc-GGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 21997 | 0.72 | 0.502528 |
Target: 5'- uAGCUUcgacGAGUUUuaCCGAGUCUAGGCCg -3' miRNA: 3'- uUCGGA----CUCAAAc-GGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 21952 | 0.72 | 0.502528 |
Target: 5'- uAGCUUcgacGAGUUUuaCCGAGUCUAGGCCg -3' miRNA: 3'- uUCGGA----CUCAAAc-GGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11731 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11081 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11601 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11536 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11471 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11406 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11341 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11276 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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28316 | 3' | -54.2 | NC_005905.1 | + | 11211 | 1.1 | 0.001577 |
Target: 5'- uAAGCCUGAGUUUGCCGAGUCCAGACCg -3' miRNA: 3'- -UUCGGACUCAAACGGCUCAGGUCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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