Results 101 - 106 of 106 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28324 | 3' | -57.2 | NC_005905.1 | + | 21953 | 0.76 | 0.192965 |
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Target: 5'- uUAGCUUcgacgaguuuuaccGaGUCUAGGCCGGCUUGGGCc -3' miRNA: 3'- gAUCGAA--------------C-CGGGUUCGGCCGAACCUG- -5' |
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| 28324 | 3' | -57.2 | NC_005905.1 | + | 21908 | 0.76 | 0.192965 |
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Target: 5'- uUAGCUUcgacgaguuuuaccGaGUCUAGGCCGGCUUGGGCc -3' miRNA: 3'- gAUCGAA--------------C-CGGGUUCGGCCGAACCUG- -5' |
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| 28324 | 3' | -57.2 | NC_005905.1 | + | 19760 | 0.75 | 0.219661 |
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Target: 5'- gCUAGCcUcgacaaauuuuaucGaGUCCAGGCCGGCUUGGGCc -3' miRNA: 3'- -GAUCGaA--------------C-CGGGUUCGGCCGAACCUG- -5' |
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| 28324 | 3' | -57.2 | NC_005905.1 | + | 22181 | 0.73 | 0.284579 |
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Target: 5'- -------aGCCCAGGCCGGCUUGGGu -3' miRNA: 3'- gaucgaacCGGGUUCGGCCGAACCUg -5' |
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| 28324 | 3' | -57.2 | NC_005905.1 | + | 17527 | 0.73 | 0.306142 |
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Target: 5'- uUGGCUUGGgCCAAGCUaGCUUcGACa -3' miRNA: 3'- gAUCGAACCgGGUUCGGcCGAAcCUG- -5' |
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| 28324 | 3' | -57.2 | NC_005905.1 | + | 17197 | 0.66 | 0.669277 |
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Target: 5'- uUGGCUUGGgCCAAGCUaGCUUcaACa -3' miRNA: 3'- gAUCGAACCgGGUUCGGcCGAAccUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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