miRNA: 3'- cCGGGUUCGGCCGGACCUG----------AGCUauu -5'
miRNA: 3'- cCGGGUUCGGCCGGACCUgAGCUauu -5'
miRNA: 3'- cCGGGUUCGGCCGGACCUG----------AGCUauu -5'
Results 101 - 103 of 103 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28325 | 3' | -58.2 | NC_005905.1 | + | 17644 | 0.76 | 0.171486 |
|
Target: 5'- aGUCCAGGCCGGCUUGGACcaagcuagcuUCGAc-- -3' miRNA: 3'- cCGGGUUCGGCCGGACCUG----------AGCUauu -5' |
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| 28325 | 3' | -58.2 | NC_005905.1 | + | 22181 | 0.8 | 0.090023 |
|
Target: 5'- aGCCCAGGCCGGCUUGGG-UCGAa-- -3' miRNA: 3'- cCGGGUUCGGCCGGACCUgAGCUauu -5' |
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| 28325 | 3' | -58.2 | NC_005905.1 | + | 17819 | 0.67 | 0.599452 |
|
Target: 5'- aGUUUAGGCCGGCUUGGGCcaagcuagcuUCGAc-- -3' miRNA: 3'- cCGGGUUCGGCCGGACCUG----------AGCUauu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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