Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28336 | 5' | -63.7 | NC_005946.1 | + | 28594 | 0.69 | 0.318613 |
Target: 5'- uGGCGUCGGCcgGGGCCG-UGGuGCCgGg -3' miRNA: 3'- cCUGCGGCCGa-CCCGGUgAUC-CGGgC- -5' |
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28336 | 5' | -63.7 | NC_005946.1 | + | 26408 | 0.66 | 0.455029 |
Target: 5'- gGGA-GCCGGUUucaGGCUGCUccuGGCCCa -3' miRNA: 3'- -CCUgCGGCCGAc--CCGGUGAu--CCGGGc -5' |
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28336 | 5' | -63.7 | NC_005946.1 | + | 20729 | 0.66 | 0.488691 |
Target: 5'- uGGACGCCguggucaccagccuGGCc-GGCCucgAGGCCCu -3' miRNA: 3'- -CCUGCGG--------------CCGacCCGGugaUCCGGGc -5' |
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28336 | 5' | -63.7 | NC_005946.1 | + | 2870 | 1.1 | 0.000334 |
Target: 5'- aGGACGCCGGCUGGGCCACUAGGCCCGa -3' miRNA: 3'- -CCUGCGGCCGACCCGGUGAUCCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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