Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28343 | 5' | -57.3 | NC_005946.1 | + | 72135 | 0.66 | 0.759397 |
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Target: 5'- cGGGGCCCUgccggUGGUGgccaUCAUGAcGGCGg -3' miRNA: 3'- cUCCUGGGG-----ACCGCg---AGUACUaCUGC- -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 44239 | 0.66 | 0.756477 |
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Target: 5'- uAGGAuccucucccagagcUCCCUGGCGCUCAcagugUGAgccACGg -3' miRNA: 3'- cUCCU--------------GGGGACCGCGAGU-----ACUac-UGC- -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 23127 | 0.67 | 0.73676 |
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Target: 5'- gGGGGucuugaugacGCCCCUGGCGcCUCAaagcuguuugggggUcugGAUGACGc -3' miRNA: 3'- -CUCC----------UGGGGACCGC-GAGU--------------A---CUACUGC- -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 72676 | 0.68 | 0.6476 |
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Target: 5'- cGGGuGACCCCgUGGgGUUCGUGAgggaGGCc -3' miRNA: 3'- -CUC-CUGGGG-ACCgCGAGUACUa---CUGc -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 41832 | 0.69 | 0.605916 |
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Target: 5'- uGGGACCCCUGGUGC-CGaccgGACc -3' miRNA: 3'- cUCCUGGGGACCGCGaGUacuaCUGc -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 102294 | 0.69 | 0.56455 |
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Target: 5'- cAGGGCCCCgcacgccagGGCGUcagagucuauggUCAUGggGGCGu -3' miRNA: 3'- cUCCUGGGGa--------CCGCG------------AGUACuaCUGC- -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 41727 | 0.7 | 0.554314 |
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Target: 5'- cGGGACCCCUGGgGC-CccGAgGACu -3' miRNA: 3'- cUCCUGGGGACCgCGaGuaCUaCUGc -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 12147 | 0.77 | 0.223369 |
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Target: 5'- aGAGGACCCUggugcacaucgUGGCcagGCUCAUGAagauUGACGg -3' miRNA: 3'- -CUCCUGGGG-----------ACCG---CGAGUACU----ACUGC- -5' |
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| 28343 | 5' | -57.3 | NC_005946.1 | + | 9130 | 1.07 | 0.00177 |
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Target: 5'- uGAGGACCCCUGGCGCUCAUGAUGACGc -3' miRNA: 3'- -CUCCUGGGGACCGCGAGUACUACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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