Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28344 | 3' | -56.9 | NC_005946.1 | + | 14914 | 0.66 | 0.814258 |
Target: 5'- gUCACGUaggccgucuuguggCCCGcuaugggagacggaGCCCUCGCcGCCGa- -3' miRNA: 3'- gAGUGUA--------------GGGU--------------UGGGAGCGuCGGCac -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 35771 | 0.66 | 0.810691 |
Target: 5'- -cCugAUCCUGGCCCUCGUAuaguccCCGUa -3' miRNA: 3'- gaGugUAGGGUUGGGAGCGUc-----GGCAc -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 42229 | 0.66 | 0.801658 |
Target: 5'- aCUC-CAUCCgGACCCUU-CAcGCCGUc -3' miRNA: 3'- -GAGuGUAGGgUUGGGAGcGU-CGGCAc -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 79079 | 0.66 | 0.764019 |
Target: 5'- --aACAcUCCGACCCUgGCGGCCu-- -3' miRNA: 3'- gagUGUaGGGUUGGGAgCGUCGGcac -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 58424 | 0.67 | 0.754281 |
Target: 5'- --gACGUCauGGCCCUCGCcaaAGCCGUa -3' miRNA: 3'- gagUGUAGggUUGGGAGCG---UCGGCAc -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 47530 | 0.67 | 0.754281 |
Target: 5'- gUCACGUCCCcgGGCaCCacCGCAGCCc-- -3' miRNA: 3'- gAGUGUAGGG--UUG-GGa-GCGUCGGcac -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 57065 | 0.67 | 0.724429 |
Target: 5'- cCUCACAuagUCCgAACCCaaaGCGGCCu-- -3' miRNA: 3'- -GAGUGU---AGGgUUGGGag-CGUCGGcac -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 67393 | 0.67 | 0.713283 |
Target: 5'- aCUCACAUCCCuaaaguccugcagGGCCCUgGUcacGGCC-UGa -3' miRNA: 3'- -GAGUGUAGGG-------------UUGGGAgCG---UCGGcAC- -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 35937 | 0.69 | 0.62099 |
Target: 5'- -cCGCcgCCagGGCCCUCGCAGCgGg- -3' miRNA: 3'- gaGUGuaGGg-UUGGGAGCGUCGgCac -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 11346 | 0.69 | 0.610551 |
Target: 5'- uCUCACGggcagaCCCGccgccACCCUCGC-GCCGg- -3' miRNA: 3'- -GAGUGUa-----GGGU-----UGGGAGCGuCGGCac -5' |
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28344 | 3' | -56.9 | NC_005946.1 | + | 9266 | 1.09 | 0.001579 |
Target: 5'- cCUCACAUCCCAACCCUCGCAGCCGUGa -3' miRNA: 3'- -GAGUGUAGGGUUGGGAGCGUCGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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