Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28350 | 5' | -54.2 | NC_005946.1 | + | 13270 | 1.07 | 0.003334 |
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Target: 5'- cAUCCUCACCCCCCACUGGAAUUACACc -3' miRNA: 3'- -UAGGAGUGGGGGGUGACCUUAAUGUG- -5' |
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| 28350 | 5' | -54.2 | NC_005946.1 | + | 11038 | 0.71 | 0.634611 |
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Target: 5'- uGUCCUCACCCUCUcccagucuGCcagGGggUgGCACa -3' miRNA: 3'- -UAGGAGUGGGGGG--------UGa--CCuuAaUGUG- -5' |
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| 28350 | 5' | -54.2 | NC_005946.1 | + | 1868 | 0.67 | 0.817367 |
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Target: 5'- cUCCUCccacuCCCCCCACUccccccacucccaGGccggUGCACa -3' miRNA: 3'- uAGGAGu----GGGGGGUGA-------------CCuua-AUGUG- -5' |
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| 28350 | 5' | -54.2 | NC_005946.1 | + | 82954 | 0.67 | 0.818275 |
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Target: 5'- -cCCUCAgCCCCgGCgGGGAUggaaaACGCu -3' miRNA: 3'- uaGGAGUgGGGGgUGaCCUUAa----UGUG- -5' |
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| 28350 | 5' | -54.2 | NC_005946.1 | + | 50956 | 0.66 | 0.869019 |
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Target: 5'- cUCC-CGCCCCCUcuacguCUGGGuUUACAa -3' miRNA: 3'- uAGGaGUGGGGGGu-----GACCUuAAUGUg -5' |
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| 28350 | 5' | -54.2 | NC_005946.1 | + | 76863 | 0.66 | 0.869019 |
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Target: 5'- gAUCC-CGCCCauCCC-CUGGAcg-ACACg -3' miRNA: 3'- -UAGGaGUGGG--GGGuGACCUuaaUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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