Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28353 | 3' | -54 | NC_005946.1 | + | 40642 | 0.66 | 0.896015 |
Target: 5'- aGACA---GGGCCAcGAGGGCGGcGGa-- -3' miRNA: 3'- -CUGUuagUCCGGU-CUCCUGCC-CUaua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 83917 | 0.66 | 0.902787 |
Target: 5'- gGACAGaCAGGgucuacUCGGAGG-CGGGGUGg -3' miRNA: 3'- -CUGUUaGUCC------GGUCUCCuGCCCUAUa -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 72851 | 0.67 | 0.866418 |
Target: 5'- aACAu---GGCCgAGGGGGCGGGGUu- -3' miRNA: 3'- cUGUuaguCCGG-UCUCCUGCCCUAua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 76325 | 0.68 | 0.777206 |
Target: 5'- aGACGugggagguAUgGGGCCAGAGGGCccaaaGGGAg-- -3' miRNA: 3'- -CUGU--------UAgUCCGGUCUCCUG-----CCCUaua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 13198 | 0.69 | 0.767329 |
Target: 5'- cGACAGgcUCaAGGCCAGGGGAgaGGcGGUGg -3' miRNA: 3'- -CUGUU--AG-UCCGGUCUCCUg-CC-CUAUa -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 72519 | 0.7 | 0.673708 |
Target: 5'- cGACAGUCccccGGCCcccGAGGAgaGGGAUAUa -3' miRNA: 3'- -CUGUUAGu---CCGGu--CUCCUg-CCCUAUA- -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 93853 | 0.7 | 0.690795 |
Target: 5'- aGGgGA-CAGGCCuuuaggagaaaauGGGGACGGGGUGUg -3' miRNA: 3'- -CUgUUaGUCCGGu------------CUCCUGCCCUAUA- -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 56877 | 0.7 | 0.716151 |
Target: 5'- uGACAG--GGGCgaAGAGGACGGGGg-- -3' miRNA: 3'- -CUGUUagUCCGg-UCUCCUGCCCUaua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 95371 | 0.71 | 0.609103 |
Target: 5'- gGACcGUCAGGcCCAGGGGcuuCGGGGg-- -3' miRNA: 3'- -CUGuUAGUCC-GGUCUCCu--GCCCUaua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 83023 | 0.71 | 0.609103 |
Target: 5'- aGACAacAUCAuGGCCGGAGGcACGGcGAg-- -3' miRNA: 3'- -CUGU--UAGU-CCGGUCUCC-UGCC-CUaua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 13679 | 0.71 | 0.630658 |
Target: 5'- uGGCGGUCAuGCCagacaGGAGGugGGGAUc- -3' miRNA: 3'- -CUGUUAGUcCGG-----UCUCCugCCCUAua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 71841 | 0.85 | 0.110563 |
Target: 5'- aGGCccgCGGGCCAGAGGACGGGAg-- -3' miRNA: 3'- -CUGuuaGUCCGGUCUCCUGCCCUaua -5' |
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28353 | 3' | -54 | NC_005946.1 | + | 13958 | 1.05 | 0.004383 |
Target: 5'- aGACAAUCAGGCCAGAGGACGGGAUAUu -3' miRNA: 3'- -CUGUUAGUCCGGUCUCCUGCCCUAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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