Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28354 | 5' | -59.8 | NC_005946.1 | + | 22173 | 0.7 | 0.408453 |
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Target: 5'- cUGC-CCUGGcCGcGGcCGCUCCCcUGACCg -3' miRNA: 3'- -ACGaGGACU-GC-CC-GUGGGGGuACUGG- -5' |
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| 28354 | 5' | -59.8 | NC_005946.1 | + | 95152 | 0.71 | 0.391429 |
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Target: 5'- aGgUCCUcGCGGGCAUCUCCAgcccgGACg -3' miRNA: 3'- aCgAGGAcUGCCCGUGGGGGUa----CUGg -5' |
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| 28354 | 5' | -59.8 | NC_005946.1 | + | 26950 | 0.71 | 0.358801 |
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Target: 5'- gUGUUCCaGGCGGGCuuCCUCUGccUGGCCa -3' miRNA: 3'- -ACGAGGaCUGCCCGu-GGGGGU--ACUGG- -5' |
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| 28354 | 5' | -59.8 | NC_005946.1 | + | 27453 | 0.71 | 0.350946 |
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Target: 5'- aGcCUCCUGAgGGGCccGCCCUC--GGCCg -3' miRNA: 3'- aC-GAGGACUgCCCG--UGGGGGuaCUGG- -5' |
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| 28354 | 5' | -59.8 | NC_005946.1 | + | 31484 | 0.72 | 0.306416 |
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Target: 5'- aUGCaaccgUCCagGACGGuGUACCugCCCAUGGCCa -3' miRNA: 3'- -ACG-----AGGa-CUGCC-CGUGG--GGGUACUGG- -5' |
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| 28354 | 5' | -59.8 | NC_005946.1 | + | 59555 | 0.76 | 0.184681 |
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Target: 5'- cGCUCCcaugGGaGCACCCaCCAUGACCa -3' miRNA: 3'- aCGAGGacugCC-CGUGGG-GGUACUGG- -5' |
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| 28354 | 5' | -59.8 | NC_005946.1 | + | 12585 | 0.78 | 0.143291 |
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Target: 5'- aGCUCCUGACGGuGU-CUCCCGUggaGACCa -3' miRNA: 3'- aCGAGGACUGCC-CGuGGGGGUA---CUGG- -5' |
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| 28354 | 5' | -59.8 | NC_005946.1 | + | 14037 | 1.12 | 0.00052 |
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Target: 5'- aUGCUCCUGACGGGCACCCCCAUGACCa -3' miRNA: 3'- -ACGAGGACUGCCCGUGGGGGUACUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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