Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28356 | 5' | -52.8 | NC_005946.1 | + | 24271 | 0.66 | 0.944792 |
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Target: 5'- -cAGGA-AAAGAGUUugUGUCGUCGAGu -3' miRNA: 3'- ucUCCUcUUUCUCAG--ACGGCAGCUCu -5' |
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| 28356 | 5' | -52.8 | NC_005946.1 | + | 48959 | 0.66 | 0.940005 |
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Target: 5'- uGGGGGAGAAGGucAGUaUGCCGUuccucaCGGGu -3' miRNA: 3'- -UCUCCUCUUUC--UCAgACGGCA------GCUCu -5' |
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| 28356 | 5' | -52.8 | NC_005946.1 | + | 83140 | 0.66 | 0.934958 |
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Target: 5'- --cGGAaaGGGAGGGUCaGCCGUCcuGAGGg -3' miRNA: 3'- ucuCCU--CUUUCUCAGaCGGCAG--CUCU- -5' |
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| 28356 | 5' | -52.8 | NC_005946.1 | + | 77311 | 0.67 | 0.924081 |
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Target: 5'- aGGAGGAcgcaGAGAGGGUC-GCagaGcUCGAGGa -3' miRNA: 3'- -UCUCCU----CUUUCUCAGaCGg--C-AGCUCU- -5' |
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| 28356 | 5' | -52.8 | NC_005946.1 | + | 12597 | 0.67 | 0.918249 |
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Target: 5'- aGGAGGuGGAGGAgcuccugacgguGUCUcCCGUgGAGAc -3' miRNA: 3'- -UCUCCuCUUUCU------------CAGAcGGCAgCUCU- -5' |
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| 28356 | 5' | -52.8 | NC_005946.1 | + | 34157 | 0.68 | 0.885218 |
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Target: 5'- aGGAGGGGAccguuGAGAGUCcacCUGUCGAcGGg -3' miRNA: 3'- -UCUCCUCU-----UUCUCAGac-GGCAGCU-CU- -5' |
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| 28356 | 5' | -52.8 | NC_005946.1 | + | 88130 | 0.73 | 0.616216 |
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Target: 5'- uGGuGGGGAccAAGAGacccUCUGCCGUgGAGGu -3' miRNA: 3'- -UCuCCUCU--UUCUC----AGACGGCAgCUCU- -5' |
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| 28356 | 5' | -52.8 | NC_005946.1 | + | 16574 | 1.1 | 0.003563 |
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Target: 5'- cAGAGGAGAAAGAGUCUGCCGUCGAGAa -3' miRNA: 3'- -UCUCCUCUUUCUCAGACGGCAGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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