miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28358 5' -53.6 NC_005946.1 + 39201 0.66 0.936325
Target:  5'- gACUUUCUGGGAGACUuucUCCCa----- -3'
miRNA:   3'- -UGAGAGGCCUUCUGAu--AGGGcuucca -5'
28358 5' -53.6 NC_005946.1 + 36480 0.67 0.872742
Target:  5'- aACUCUCCGGggGuCcugGUCCCaaacGGcAGGg -3'
miRNA:   3'- -UGAGAGGCCuuCuGa--UAGGG----CU-UCCa -5'
28358 5' -53.6 NC_005946.1 + 39004 0.67 0.894458
Target:  5'- gACUUUCUGGGAGACUuucUCaCUGGAGa- -3'
miRNA:   3'- -UGAGAGGCCUUCUGAu--AG-GGCUUCca -5'
28358 5' -53.6 NC_005946.1 + 90681 0.67 0.901206
Target:  5'- gGCUCUCCugagcagcGGGAGGCccuugGUCUCG-AGGUc -3'
miRNA:   3'- -UGAGAGG--------CCUUCUGa----UAGGGCuUCCA- -5'
28358 5' -53.6 NC_005946.1 + 23066 0.74 0.506431
Target:  5'- ----cCCGGAGGACccggagGUCCCGGAGGUc -3'
miRNA:   3'- ugagaGGCCUUCUGa-----UAGGGCUUCCA- -5'
28358 5' -53.6 NC_005946.1 + 19963 1.07 0.004566
Target:  5'- gACUCUCCGGAAGACUAUCCCGAAGGUc -3'
miRNA:   3'- -UGAGAGGCCUUCUGAUAGGGCUUCCA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.