Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28359 | 5' | -51.2 | NC_005946.1 | + | 77347 | 0.66 | 0.977571 |
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Target: 5'- -cGUgGGuCGUGACCAAGGaccucauCGAg -3' miRNA: 3'- caCAgCCuGUACUGGUUCCgaau---GCU- -5' |
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| 28359 | 5' | -51.2 | NC_005946.1 | + | 14575 | 0.66 | 0.977571 |
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Target: 5'- -cGUCGGACGUuGCCAugaggaGGGCcguccaggACGAc -3' miRNA: 3'- caCAGCCUGUAcUGGU------UCCGaa------UGCU- -5' |
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| 28359 | 5' | -51.2 | NC_005946.1 | + | 49759 | 0.66 | 0.972117 |
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Target: 5'- -aGUUGGGCAccagggaGGCCGAGGCgUACu- -3' miRNA: 3'- caCAGCCUGUa------CUGGUUCCGaAUGcu -5' |
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| 28359 | 5' | -51.2 | NC_005946.1 | + | 56541 | 0.67 | 0.962238 |
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Target: 5'- cGUGUCGuaGACGUGAUCGuccacuuucacgGGGCccccUGCGAa -3' miRNA: 3'- -CACAGC--CUGUACUGGU------------UCCGa---AUGCU- -5' |
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| 28359 | 5' | -51.2 | NC_005946.1 | + | 99100 | 0.67 | 0.954437 |
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Target: 5'- cGUGcCaaaaGACAuUGACCAAGGCgcccucgUACGAa -3' miRNA: 3'- -CACaGc---CUGU-ACUGGUUCCGa------AUGCU- -5' |
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| 28359 | 5' | -51.2 | NC_005946.1 | + | 20275 | 1.09 | 0.005099 |
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Target: 5'- gGUGUCGGACAUGACCAAGGCUUACGAg -3' miRNA: 3'- -CACAGCCUGUACUGGUUCCGAAUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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