miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28361 3' -54.5 NC_005946.1 + 95445 0.66 0.877704
Target:  5'- uGGGAGacccgccgcAGGCUCgagaGCAGCaGCCGgcgCGg -3'
miRNA:   3'- gCCCUU---------UCUGAGaa--CGUCG-CGGCa--GU- -5'
28361 3' -54.5 NC_005946.1 + 101428 0.66 0.870166
Target:  5'- aGGGAGAGGCggcgUGCAGggaUGCCGcCu -3'
miRNA:   3'- gCCCUUUCUGaga-ACGUC---GCGGCaGu -5'
28361 3' -54.5 NC_005946.1 + 9691 0.67 0.854407
Target:  5'- uCGGGGGuGAg---UGCGGCGCaCGUCAc -3'
miRNA:   3'- -GCCCUUuCUgagaACGUCGCG-GCAGU- -5'
28361 3' -54.5 NC_005946.1 + 13181 0.67 0.846199
Target:  5'- gGGGAGAGGCggugGCAGUcaCCGUCc -3'
miRNA:   3'- gCCCUUUCUGagaaCGUCGc-GGCAGu -5'
28361 3' -54.5 NC_005946.1 + 20850 0.68 0.783353
Target:  5'- aGGGcAAAGGCUgcgGCAGaCGCCGUg- -3'
miRNA:   3'- gCCC-UUUCUGAgaaCGUC-GCGGCAgu -5'
28361 3' -54.5 NC_005946.1 + 21482 0.69 0.763938
Target:  5'- aGGGAccuGGACUC-UGCGGUagagGCCGUg- -3'
miRNA:   3'- gCCCUu--UCUGAGaACGUCG----CGGCAgu -5'
28361 3' -54.5 NC_005946.1 + 22138 1.09 0.002512
Target:  5'- gCGGGAAAGACUCUUGCAGCGCCGUCAg -3'
miRNA:   3'- -GCCCUUUCUGAGAACGUCGCGGCAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.