Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28361 | 3' | -54.5 | NC_005946.1 | + | 95445 | 0.66 | 0.877704 |
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Target: 5'- uGGGAGacccgccgcAGGCUCgagaGCAGCaGCCGgcgCGg -3' miRNA: 3'- gCCCUU---------UCUGAGaa--CGUCG-CGGCa--GU- -5' |
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| 28361 | 3' | -54.5 | NC_005946.1 | + | 101428 | 0.66 | 0.870166 |
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Target: 5'- aGGGAGAGGCggcgUGCAGggaUGCCGcCu -3' miRNA: 3'- gCCCUUUCUGaga-ACGUC---GCGGCaGu -5' |
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| 28361 | 3' | -54.5 | NC_005946.1 | + | 9691 | 0.67 | 0.854407 |
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Target: 5'- uCGGGGGuGAg---UGCGGCGCaCGUCAc -3' miRNA: 3'- -GCCCUUuCUgagaACGUCGCG-GCAGU- -5' |
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| 28361 | 3' | -54.5 | NC_005946.1 | + | 13181 | 0.67 | 0.846199 |
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Target: 5'- gGGGAGAGGCggugGCAGUcaCCGUCc -3' miRNA: 3'- gCCCUUUCUGagaaCGUCGc-GGCAGu -5' |
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| 28361 | 3' | -54.5 | NC_005946.1 | + | 20850 | 0.68 | 0.783353 |
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Target: 5'- aGGGcAAAGGCUgcgGCAGaCGCCGUg- -3' miRNA: 3'- gCCC-UUUCUGAgaaCGUC-GCGGCAgu -5' |
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| 28361 | 3' | -54.5 | NC_005946.1 | + | 21482 | 0.69 | 0.763938 |
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Target: 5'- aGGGAccuGGACUC-UGCGGUagagGCCGUg- -3' miRNA: 3'- gCCCUu--UCUGAGaACGUCG----CGGCAgu -5' |
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| 28361 | 3' | -54.5 | NC_005946.1 | + | 22138 | 1.09 | 0.002512 |
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Target: 5'- gCGGGAAAGACUCUUGCAGCGCCGUCAg -3' miRNA: 3'- -GCCCUUUCUGAGAACGUCGCGGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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