Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28364 | 5' | -60.7 | NC_005946.1 | + | 97868 | 0.66 | 0.669734 |
Target: 5'- gGCUCCgUCCUGGcCUguGGCC-GGGGCg -3' miRNA: 3'- gUGAGG-AGGGCCaGG--CUGGaCCCUGg -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 24052 | 0.66 | 0.649731 |
Target: 5'- aGCUCCcuuUCCCcaccGUCCGAcgucCCUGGGuacgaACCu -3' miRNA: 3'- gUGAGG---AGGGc---CAGGCU----GGACCC-----UGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 22465 | 0.66 | 0.619645 |
Target: 5'- aUugUCCUgCCGGUCCuaggguuCCUgguGGGAUCc -3' miRNA: 3'- -GugAGGAgGGCCAGGcu-----GGA---CCCUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 10244 | 0.67 | 0.579689 |
Target: 5'- -cCUCCUCuacgCUGGUCaCcGCCgGGGACCu -3' miRNA: 3'- guGAGGAG----GGCCAG-GcUGGaCCCUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 61862 | 0.68 | 0.550076 |
Target: 5'- -cCUCCUCCCGacGcCCuGCCcaGGGACCu -3' miRNA: 3'- guGAGGAGGGC--CaGGcUGGa-CCCUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 35613 | 0.68 | 0.520945 |
Target: 5'- aCACgg-UCCCGGcCgCGGCCUGGGcuuCCa -3' miRNA: 3'- -GUGaggAGGGCCaG-GCUGGACCCu--GG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 14994 | 0.69 | 0.492436 |
Target: 5'- aGC-CCUCaaaaCGGUCCGACCccauguuggagGGGGCg -3' miRNA: 3'- gUGaGGAGg---GCCAGGCUGGa----------CCCUGg -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 62094 | 0.69 | 0.471994 |
Target: 5'- uCAgaCCUCaCCagaaaggGGUCCugaaggaGGCCUGGGGCCa -3' miRNA: 3'- -GUgaGGAG-GG-------CCAGG-------CUGGACCCUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 67996 | 0.69 | 0.446614 |
Target: 5'- gCACgUUgUagCCGGUgCGACCUGGGGCUu -3' miRNA: 3'- -GUG-AGgAg-GGCCAgGCUGGACCCUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 28760 | 0.7 | 0.403266 |
Target: 5'- uGCUCCUUCUGGUa-GGCCaUGGuGGCCu -3' miRNA: 3'- gUGAGGAGGGCCAggCUGG-ACC-CUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 74501 | 0.72 | 0.317838 |
Target: 5'- -cCUCCUCCuCGGcCuCGACCUGGuuGACUg -3' miRNA: 3'- guGAGGAGG-GCCaG-GCUGGACC--CUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 13764 | 0.73 | 0.28835 |
Target: 5'- ---gCCUUCCaGUCCGacgcauacgccucgGCCUGGGACCa -3' miRNA: 3'- gugaGGAGGGcCAGGC--------------UGGACCCUGG- -5' |
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28364 | 5' | -60.7 | NC_005946.1 | + | 22746 | 1.11 | 0.00063 |
Target: 5'- aCACUCCUCCCGGUCCGACCUGGGACCu -3' miRNA: 3'- -GUGAGGAGGGCCAGGCUGGACCCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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