Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28365 | 5' | -58.9 | NC_005946.1 | + | 63087 | 0.66 | 0.690925 |
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Target: 5'- cGuCGCUCCUGGC-CCUCAAGaucCUGc -3' miRNA: 3'- aCuGCGGGGACCGcGGAGUUUc--GACa -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 73241 | 0.66 | 0.670276 |
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Target: 5'- gGACGCCaCCgGGCcCCUCAucGCc-- -3' miRNA: 3'- aCUGCGG-GGaCCGcGGAGUuuCGaca -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 3926 | 0.66 | 0.65054 |
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Target: 5'- aUGACGUCCgUGGCGCCacucaugucaaccgaCAGGGCc-- -3' miRNA: 3'- -ACUGCGGGgACCGCGGa--------------GUUUCGaca -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 44346 | 0.67 | 0.604723 |
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Target: 5'- uUGcUGCCCCUGaucuugagacccuuGCGCUUgAGGGCUGg -3' miRNA: 3'- -ACuGCGGGGAC--------------CGCGGAgUUUCGACa -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 34866 | 0.68 | 0.546056 |
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Target: 5'- gGACagGUCCCUGGCcaGCCUgAAGGCgGg -3' miRNA: 3'- aCUG--CGGGGACCG--CGGAgUUUCGaCa -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 24012 | 0.71 | 0.377695 |
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Target: 5'- cUGGCGUUCCUGGUGCCguaCAGGGUg-- -3' miRNA: 3'- -ACUGCGGGGACCGCGGa--GUUUCGaca -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 20728 | 0.72 | 0.314871 |
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Target: 5'- gGACGCCguggucaccagCCUGGCcgGCCUCGAGGCccUGg -3' miRNA: 3'- aCUGCGG-----------GGACCG--CGGAGUUUCG--ACa -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 23157 | 0.78 | 0.141663 |
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Target: 5'- cUGGcCGCUCC-GGaCGCCUCAAAGCUGUu -3' miRNA: 3'- -ACU-GCGGGGaCC-GCGGAGUUUCGACA- -5' |
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| 28365 | 5' | -58.9 | NC_005946.1 | + | 23117 | 1.07 | 0.001237 |
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Target: 5'- aUGACGCCCCUGGCGCCUCAAAGCUGUu -3' miRNA: 3'- -ACUGCGGGGACCGCGGAGUUUCGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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