Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28366 | 3' | -49.7 | NC_005946.1 | + | 96511 | 0.66 | 0.991672 |
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Target: 5'- gCGAGAGccugcaGGAGCCUcuGAAccGAgGGUGGg -3' miRNA: 3'- aGCUCUCa-----CUUCGGG--CUU--UUgUCACC- -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 26754 | 0.66 | 0.990427 |
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Target: 5'- -gGGGAGUcucGAcgucCCCGAAGGCAGUGu -3' miRNA: 3'- agCUCUCA---CUuc--GGGCUUUUGUCACc -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 54709 | 0.67 | 0.98393 |
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Target: 5'- -gGAGccuGUGAAGCCCaAAAAgGGUGu -3' miRNA: 3'- agCUCu--CACUUCGGGcUUUUgUCACc -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 103056 | 0.68 | 0.974538 |
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Target: 5'- --uGGAGUgGGAGCCaGggGGCuAGUGGg -3' miRNA: 3'- agcUCUCA-CUUCGGgCuuUUG-UCACC- -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 59838 | 0.69 | 0.957461 |
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Target: 5'- aCGAGAGcgucuccUGGAGCuuGAcgGCcGUGGu -3' miRNA: 3'- aGCUCUC-------ACUUCGggCUuuUGuCACC- -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 16236 | 0.69 | 0.953788 |
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Target: 5'- gUGAGcAGcGGcauGGCCCaGGAGACGGUGGc -3' miRNA: 3'- aGCUC-UCaCU---UCGGG-CUUUUGUCACC- -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 66899 | 0.72 | 0.876844 |
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Target: 5'- -aGAGGGUGucuGCCCGAG---AGUGGc -3' miRNA: 3'- agCUCUCACuu-CGGGCUUuugUCACC- -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 54012 | 0.73 | 0.826957 |
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Target: 5'- -gGAGAGUGuuuGGCCUGG--ACAGUGa -3' miRNA: 3'- agCUCUCACu--UCGGGCUuuUGUCACc -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 76402 | 0.75 | 0.718506 |
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Target: 5'- aUGAGGGgccuaGAGGCCCccgGAGCGGUGGg -3' miRNA: 3'- aGCUCUCa----CUUCGGGcu-UUUGUCACC- -5' |
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| 28366 | 3' | -49.7 | NC_005946.1 | + | 24444 | 1.13 | 0.004763 |
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Target: 5'- gUCGAGAGUGAAGCCCGAAAACAGUGGg -3' miRNA: 3'- -AGCUCUCACUUCGGGCUUUUGUCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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