Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28368 | 3' | -57.7 | NC_005946.1 | + | 21604 | 0.66 | 0.721857 |
Target: 5'- --cCCUGUCgGCCGCCa-UCUCCAAc -3' miRNA: 3'- auaGGACAGaCGGUGGcgGGAGGUUu -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 21749 | 0.66 | 0.711533 |
Target: 5'- --cCCUGUUUGUCAUgGCCCUgagcgcgagccCCGAAu -3' miRNA: 3'- auaGGACAGACGGUGgCGGGA-----------GGUUU- -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 96941 | 0.66 | 0.701135 |
Target: 5'- cGUC---UCUGCCACUGUCCUCUGAc -3' miRNA: 3'- aUAGgacAGACGGUGGCGGGAGGUUu -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 48418 | 0.66 | 0.690675 |
Target: 5'- gAUCCUGccCUGCCucCUGgCCUCCGc- -3' miRNA: 3'- aUAGGACa-GACGGu-GGCgGGAGGUuu -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 19596 | 0.66 | 0.690675 |
Target: 5'- --aCCUGUCUGCCGCagcgucuguccuUGCCuCUgCCGAc -3' miRNA: 3'- auaGGACAGACGGUG------------GCGG-GA-GGUUu -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 9621 | 0.66 | 0.680163 |
Target: 5'- cGUCCUcGUCauCCGCCGCcuccgCCUCCAGGa -3' miRNA: 3'- aUAGGA-CAGacGGUGGCG-----GGAGGUUU- -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 69938 | 0.66 | 0.669608 |
Target: 5'- gGUgCcGUCUGCCACCGucucCCCUCUc-- -3' miRNA: 3'- aUAgGaCAGACGGUGGC----GGGAGGuuu -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 68179 | 0.68 | 0.595323 |
Target: 5'- gGUCCa--CUGCCACgaCGCCCUCCc-- -3' miRNA: 3'- aUAGGacaGACGGUG--GCGGGAGGuuu -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 27554 | 0.68 | 0.563753 |
Target: 5'- cAUCCUcGUCUGUCGCCGaguCCUCUu-- -3' miRNA: 3'- aUAGGA-CAGACGGUGGCg--GGAGGuuu -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 34212 | 0.69 | 0.492183 |
Target: 5'- gGUCCUGUUggaucccgUGCCcccuaCGUCCUCCAGAa -3' miRNA: 3'- aUAGGACAG--------ACGGug---GCGGGAGGUUU- -5' |
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28368 | 3' | -57.7 | NC_005946.1 | + | 26238 | 1.04 | 0.002033 |
Target: 5'- cUAUCCUGUCUGCCACCGCCCUCCAAAa -3' miRNA: 3'- -AUAGGACAGACGGUGGCGGGAGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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