Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28369 | 5' | -54.5 | NC_005946.1 | + | 43763 | 0.66 | 0.858694 |
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Target: 5'- -gGGGGaAGAGUaaccgaaccagcccUUGCAGGCGUagucucUCAGUu -3' miRNA: 3'- caCCCC-UCUCA--------------AACGUCUGCA------GGUCA- -5' |
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| 28369 | 5' | -54.5 | NC_005946.1 | + | 13658 | 0.67 | 0.818649 |
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Target: 5'- gGUGGGGAucGgcUGCGGAgG-CCAGg -3' miRNA: 3'- -CACCCCUcuCaaACGUCUgCaGGUCa -5' |
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| 28369 | 5' | -54.5 | NC_005946.1 | + | 99571 | 0.68 | 0.809573 |
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Target: 5'- -aGGGGAGAaucUUGUAGugaAUGUCCAGa -3' miRNA: 3'- caCCCCUCUca-AACGUC---UGCAGGUCa -5' |
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| 28369 | 5' | -54.5 | NC_005946.1 | + | 4760 | 0.68 | 0.780367 |
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Target: 5'- gGUGGGGAcAGUguucaggaaugggUUGguGcCGUCCAGg -3' miRNA: 3'- -CACCCCUcUCA-------------AACguCuGCAGGUCa -5' |
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| 28369 | 5' | -54.5 | NC_005946.1 | + | 13182 | 0.7 | 0.689868 |
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Target: 5'- -aGGGGAGAGgcggugGCAGucacCGUCCAc- -3' miRNA: 3'- caCCCCUCUCaaa---CGUCu---GCAGGUca -5' |
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| 28369 | 5' | -54.5 | NC_005946.1 | + | 28876 | 1.07 | 0.00323 |
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Target: 5'- cGUGGGGAGAGUUUGCAGACGUCCAGUc -3' miRNA: 3'- -CACCCCUCUCAAACGUCUGCAGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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