Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28372 | 5' | -58.6 | NC_005946.1 | + | 61249 | 0.66 | 0.703729 |
Target: 5'- aGAGGaCCgGcCUGUGCCUGGcGgcGGAc -3' miRNA: 3'- gUUCCaGGgC-GACGUGGACC-CauCCU- -5' |
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28372 | 5' | -58.6 | NC_005946.1 | + | 71045 | 0.66 | 0.703729 |
Target: 5'- aCGAGGUCCCGgUGUGCaaagagUGcGGcAGGAu -3' miRNA: 3'- -GUUCCAGGGCgACGUGg-----AC-CCaUCCU- -5' |
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28372 | 5' | -58.6 | NC_005946.1 | + | 49625 | 0.66 | 0.693541 |
Target: 5'- aAGGGUCCCGggGCcucgaACCUGGGg---- -3' miRNA: 3'- gUUCCAGGGCgaCG-----UGGACCCauccu -5' |
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28372 | 5' | -58.6 | NC_005946.1 | + | 76364 | 0.67 | 0.631626 |
Target: 5'- --cGGUCCCaGCgggGCGCCUGGaucucAGGGc -3' miRNA: 3'- guuCCAGGG-CGa--CGUGGACCca---UCCU- -5' |
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28372 | 5' | -58.6 | NC_005946.1 | + | 97046 | 0.69 | 0.539249 |
Target: 5'- gCAGGGcgUUGCUGCugCUGGGcAGGGc -3' miRNA: 3'- -GUUCCagGGCGACGugGACCCaUCCU- -5' |
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28372 | 5' | -58.6 | NC_005946.1 | + | 97004 | 0.69 | 0.539249 |
Target: 5'- gCAGGGcgUUGCUGCugCUGGGcAGGGc -3' miRNA: 3'- -GUUCCagGGCGACGugGACCCaUCCU- -5' |
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28372 | 5' | -58.6 | NC_005946.1 | + | 7048 | 0.69 | 0.519256 |
Target: 5'- -uAGGUCUggugugCGCgUGCGuCUUGGGUGGGAu -3' miRNA: 3'- guUCCAGG------GCG-ACGU-GGACCCAUCCU- -5' |
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28372 | 5' | -58.6 | NC_005946.1 | + | 35283 | 1.08 | 0.001253 |
Target: 5'- uCAAGGUCCCGCUGCACCUGGGUAGGAc -3' miRNA: 3'- -GUUCCAGGGCGACGUGGACCCAUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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