Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28373 | 3' | -65 | NC_005946.1 | + | 100895 | 0.66 | 0.443826 |
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Target: 5'- uCGGUCuuGGCC--GGUCUGacGGCUgCCa -3' miRNA: 3'- -GCCAGggCCGGcgCCGGAC--CCGAaGG- -5' |
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| 28373 | 3' | -65 | NC_005946.1 | + | 86991 | 0.66 | 0.443826 |
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Target: 5'- gGGUUCgGcGCC-CGGCCUGGGg-UCa -3' miRNA: 3'- gCCAGGgC-CGGcGCCGGACCCgaAGg -5' |
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| 28373 | 3' | -65 | NC_005946.1 | + | 88252 | 0.67 | 0.401587 |
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Target: 5'- gGGUCCC-GCCGgagaCGGCCUGGucgucGCgcagUCUg -3' miRNA: 3'- gCCAGGGcCGGC----GCCGGACC-----CGa---AGG- -5' |
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| 28373 | 3' | -65 | NC_005946.1 | + | 59500 | 0.67 | 0.385422 |
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Target: 5'- gGGUCCaGGCCcgaGGCCUuGGCgucUCCc -3' miRNA: 3'- gCCAGGgCCGGcg-CCGGAcCCGa--AGG- -5' |
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| 28373 | 3' | -65 | NC_005946.1 | + | 69804 | 0.67 | 0.377502 |
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Target: 5'- uGGUCUCGGCCuuGGCCU---UUUCCa -3' miRNA: 3'- gCCAGGGCCGGcgCCGGAcccGAAGG- -5' |
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| 28373 | 3' | -65 | NC_005946.1 | + | 97863 | 0.72 | 0.187359 |
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Target: 5'- -cGUCCUGGCCuGUGGCCgGGGCg--- -3' miRNA: 3'- gcCAGGGCCGG-CGCCGGaCCCGaagg -5' |
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| 28373 | 3' | -65 | NC_005946.1 | + | 35611 | 1.11 | 0.000226 |
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Target: 5'- aCGGUCCCGGCCGCGGCCUGGGCUUCCa -3' miRNA: 3'- -GCCAGGGCCGGCGCCGGACCCGAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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