Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28383 | 3' | -56.1 | NC_005946.1 | + | 56696 | 0.68 | 0.721997 |
Target: 5'- cUCCACAGGGUCUgaGGGUacaUGGCGUc-- -3' miRNA: 3'- uAGGUGUCUCAGA--CCCA---GCUGCAccu -5' |
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28383 | 3' | -56.1 | NC_005946.1 | + | 9342 | 0.71 | 0.555474 |
Target: 5'- cUCCACAGuccacAGUCaGGGUgGACG-GGAg -3' miRNA: 3'- uAGGUGUC-----UCAGaCCCAgCUGCaCCU- -5' |
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28383 | 3' | -56.1 | NC_005946.1 | + | 103822 | 0.75 | 0.35121 |
Target: 5'- -gCgGCAGAGUCUGGGUgGAgGgUGGAc -3' miRNA: 3'- uaGgUGUCUCAGACCCAgCUgC-ACCU- -5' |
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28383 | 3' | -56.1 | NC_005946.1 | + | 47121 | 1.06 | 0.002684 |
Target: 5'- cAUCCACAGAGUCUGGGUCGACGUGGAc -3' miRNA: 3'- -UAGGUGUCUCAGACCCAGCUGCACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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