Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28385 | 5' | -57.1 | NC_005946.1 | + | 6179 | 0.66 | 0.819125 |
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Target: 5'- uGGGUCUugucguuuaucCUGaUugUCCCGcucaggcuaaacuuuUGGUCCUUg -3' miRNA: 3'- -CCCAGA-----------GACcAugAGGGC---------------ACCAGGAG- -5' |
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| 28385 | 5' | -57.1 | NC_005946.1 | + | 27176 | 0.66 | 0.815634 |
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Target: 5'- uGGGccUCaUCUGGUugUUCCagaUGGUCCa- -3' miRNA: 3'- -CCC--AG-AGACCAugAGGGc--ACCAGGag -5' |
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| 28385 | 5' | -57.1 | NC_005946.1 | + | 42396 | 0.67 | 0.720995 |
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Target: 5'- gGGGUCUUUGGgaccucuguCUCCCG-GGaCCa- -3' miRNA: 3'- -CCCAGAGACCau-------GAGGGCaCCaGGag -5' |
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| 28385 | 5' | -57.1 | NC_005946.1 | + | 36499 | 0.69 | 0.628873 |
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Target: 5'- gGGGUCaaaccagCUGGggaACUCuCCGggGGUCCUg -3' miRNA: 3'- -CCCAGa------GACCa--UGAG-GGCa-CCAGGAg -5' |
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| 28385 | 5' | -57.1 | NC_005946.1 | + | 68977 | 0.74 | 0.347232 |
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Target: 5'- cGGUCUuuguccccagCUGGUucuugagggcgGCUCUgGUGGUCCUCa -3' miRNA: 3'- cCCAGA----------GACCA-----------UGAGGgCACCAGGAG- -5' |
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| 28385 | 5' | -57.1 | NC_005946.1 | + | 48543 | 1.12 | 0.001041 |
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Target: 5'- gGGGUCUCUGGUACUCCCGUGGUCCUCa -3' miRNA: 3'- -CCCAGAGACCAUGAGGGCACCAGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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