Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28386 | 5' | -57.8 | NC_005946.1 | + | 49289 | 0.67 | 0.725613 |
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Target: 5'- cCCGUCUCCaccgacCUCCUgUagcccgacgggacgaACCUGgagCCCAGg -3' miRNA: 3'- -GGUAGAGG------GAGGAgG---------------UGGACa--GGGUC- -5' |
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| 28386 | 5' | -57.8 | NC_005946.1 | + | 80320 | 0.66 | 0.751133 |
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Target: 5'- gCAUCccUCCCUCUUCCGC--GUgCCAGu -3' miRNA: 3'- gGUAG--AGGGAGGAGGUGgaCAgGGUC- -5' |
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| 28386 | 5' | -57.8 | NC_005946.1 | + | 24055 | 0.66 | 0.751133 |
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Target: 5'- uCCAgCUCCCuuUCC-CCACCguccgacGUCCCuGg -3' miRNA: 3'- -GGUaGAGGG--AGGaGGUGGa------CAGGGuC- -5' |
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| 28386 | 5' | -57.8 | NC_005946.1 | + | 10671 | 0.66 | 0.786176 |
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Target: 5'- uCCAggaggUCCCUCCUgacgaacgagcaccCCACCagGUCCCu- -3' miRNA: 3'- -GGUag---AGGGAGGA--------------GGUGGa-CAGGGuc -5' |
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| 28386 | 5' | -57.8 | NC_005946.1 | + | 96456 | 0.66 | 0.78894 |
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Target: 5'- cCCuUCUUCUUUCUCCuCUuggGUCCCGGu -3' miRNA: 3'- -GGuAGAGGGAGGAGGuGGa--CAGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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