Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28386 | 5' | -57.8 | NC_005946.1 | + | 80394 | 0.69 | 0.615511 |
Target: 5'- gCCGUCUCCCgcaaagaggaCCAgCCUGUCCCu- -3' miRNA: 3'- -GGUAGAGGGagga------GGU-GGACAGGGuc -5' |
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28386 | 5' | -57.8 | NC_005946.1 | + | 89216 | 0.76 | 0.251805 |
Target: 5'- aCgGUC-CCCUCCUCgACCUuUCCCGGa -3' miRNA: 3'- -GgUAGaGGGAGGAGgUGGAcAGGGUC- -5' |
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28386 | 5' | -57.8 | NC_005946.1 | + | 94093 | 0.67 | 0.711632 |
Target: 5'- cCCAgCUCCCgccggCCUCCugUU-UCCCAa -3' miRNA: 3'- -GGUaGAGGGa----GGAGGugGAcAGGGUc -5' |
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28386 | 5' | -57.8 | NC_005946.1 | + | 96333 | 0.69 | 0.619627 |
Target: 5'- gCCAuucucUCUUCCUCCUCagacauCCUGUCCa-- -3' miRNA: 3'- -GGU-----AGAGGGAGGAGgu----GGACAGGguc -5' |
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28386 | 5' | -57.8 | NC_005946.1 | + | 96456 | 0.66 | 0.78894 |
Target: 5'- cCCuUCUUCUUUCUCCuCUuggGUCCCGGu -3' miRNA: 3'- -GGuAGAGGGAGGAGGuGGa--CAGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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