Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28388 | 5' | -55.7 | NC_005946.1 | + | 47440 | 0.66 | 0.8448 |
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Target: 5'- aAGAAGacuGCGCcuucGGCGUGAGCGGu -3' miRNA: 3'- -UCUUCcu-CGCGuuccUCGCACUCGUC- -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 36416 | 0.68 | 0.742246 |
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Target: 5'- cGGAAGGGGCacGgAGGGAcGCGgaaccugcGGGCAGg -3' miRNA: 3'- -UCUUCCUCG--CgUUCCU-CGCa-------CUCGUC- -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 8024 | 0.68 | 0.732098 |
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Target: 5'- uGAu--GGCGCAGGGAuCGUGAGCGu -3' miRNA: 3'- uCUuccUCGCGUUCCUcGCACUCGUc -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 71148 | 0.68 | 0.732098 |
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Target: 5'- aGGGAGGGGgGCAuaaagaucgGGGAGaugGAGCAa -3' miRNA: 3'- -UCUUCCUCgCGU---------UCCUCgcaCUCGUc -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 88458 | 0.68 | 0.690675 |
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Target: 5'- aAGAAGGAcGUGCuAGGAGgG-GAcGCAGu -3' miRNA: 3'- -UCUUCCU-CGCGuUCCUCgCaCU-CGUC- -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 43468 | 0.69 | 0.680163 |
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Target: 5'- uGAAGGuGUGUucAGGAGCGgauAGCAGu -3' miRNA: 3'- uCUUCCuCGCGu-UCCUCGCac-UCGUC- -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 12073 | 0.69 | 0.669608 |
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Target: 5'- uGAGGGAGaUG-AGGGAGCuGgcgGAGCAGg -3' miRNA: 3'- uCUUCCUC-GCgUUCCUCG-Ca--CUCGUC- -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 2337 | 0.72 | 0.482286 |
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Target: 5'- gAGggGGguaaagaacacGGC-CGGGGAGCucGUGAGCAGg -3' miRNA: 3'- -UCuuCC-----------UCGcGUUCCUCG--CACUCGUC- -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 91652 | 0.74 | 0.380643 |
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Target: 5'- uGAAGGAcuuuaggucGgGCAGGGcAGCGUGAGgCAGg -3' miRNA: 3'- uCUUCCU---------CgCGUUCC-UCGCACUC-GUC- -5' |
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| 28388 | 5' | -55.7 | NC_005946.1 | + | 50876 | 1.08 | 0.002033 |
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Target: 5'- gAGAAGGAGCGCAAGGAGCGUGAGCAGc -3' miRNA: 3'- -UCUUCCUCGCGUUCCUCGCACUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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