Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28398 | 3' | -65 | NC_005946.1 | + | 60149 | 0.66 | 0.408289 |
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Target: 5'- cGGAGcCGGUCAGgGCUGCCgUGGUg- -3' miRNA: 3'- -UCUCcGCCAGUCgCGGCGGgGUCGgu -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 13178 | 0.66 | 0.391667 |
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Target: 5'- gAGAGGCGGUggCAGUcaCCGUCCacaGGCUg -3' miRNA: 3'- -UCUCCGCCA--GUCGc-GGCGGGg--UCGGu -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 15923 | 0.66 | 0.367574 |
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Target: 5'- cGAGGCGGUCguggccauggaGGcCGCCGaUCCCAcCCu -3' miRNA: 3'- uCUCCGCCAG-----------UC-GCGGC-GGGGUcGGu -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 53228 | 0.67 | 0.32181 |
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Target: 5'- cGGAgGGUGGacgCAGUcaagcccGCCGCCCCAGaCAa -3' miRNA: 3'- -UCU-CCGCCa--GUCG-------CGGCGGGGUCgGU- -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 64278 | 0.68 | 0.281083 |
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Target: 5'- uAGuAGGCGGUgucaguccacaCGGCgucagagGCCGCCUgAGCCAu -3' miRNA: 3'- -UC-UCCGCCA-----------GUCG-------CGGCGGGgUCGGU- -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 75002 | 0.69 | 0.239429 |
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Target: 5'- ---cGCGGcucCAGCGCUGCCCCugaugaGGCCAg -3' miRNA: 3'- ucucCGCCa--GUCGCGGCGGGG------UCGGU- -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 40740 | 0.7 | 0.212568 |
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Target: 5'- uGGuAGGCGG-CGGCGCCGCUagCGGCg- -3' miRNA: 3'- -UC-UCCGCCaGUCGCGGCGGg-GUCGgu -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 94023 | 0.72 | 0.1625 |
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Target: 5'- aGGAGGCuGGUgauugcCAGgGCCcgcagggacGCCCCGGCCGu -3' miRNA: 3'- -UCUCCG-CCA------GUCgCGG---------CGGGGUCGGU- -5' |
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| 28398 | 3' | -65 | NC_005946.1 | + | 60377 | 1.07 | 0.000366 |
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Target: 5'- cAGAGGCGGUCAGCGCCGCCCCAGCCAc -3' miRNA: 3'- -UCUCCGCCAGUCGCGGCGGGGUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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