Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28401 | 5' | -57.7 | NC_005946.1 | + | 60381 | 0.66 | 0.767381 |
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Target: 5'- -aGGGcagagGCGGucagcGCCGCCccagccacUCCCGCUCc -3' miRNA: 3'- gaCCCua---CGUU-----UGGCGG--------AGGGCGAGu -5' |
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| 28401 | 5' | -57.7 | NC_005946.1 | + | 43452 | 0.66 | 0.757697 |
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Target: 5'- -aGcGGAUaGCAGucACCuCCUCCUGCUCc -3' miRNA: 3'- gaC-CCUA-CGUU--UGGcGGAGGGCGAGu -5' |
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| 28401 | 5' | -57.7 | NC_005946.1 | + | 79381 | 0.66 | 0.716893 |
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Target: 5'- aUGGuGAaGUAGACUgccgucuGCCUCCUGUUCAu -3' miRNA: 3'- gACC-CUaCGUUUGG-------CGGAGGGCGAGU- -5' |
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| 28401 | 5' | -57.7 | NC_005946.1 | + | 19789 | 0.67 | 0.666521 |
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Target: 5'- gUGGaGAaGCAGGCCGuCCUCCUGUc-- -3' miRNA: 3'- gACC-CUaCGUUUGGC-GGAGGGCGagu -5' |
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| 28401 | 5' | -57.7 | NC_005946.1 | + | 72273 | 0.68 | 0.645699 |
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Target: 5'- -gGGGAaGCAGGCCauagGCUUCCCcgccguaaacuGCUCAg -3' miRNA: 3'- gaCCCUaCGUUUGG----CGGAGGG-----------CGAGU- -5' |
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| 28401 | 5' | -57.7 | NC_005946.1 | + | 62153 | 1.08 | 0.001398 |
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Target: 5'- cCUGGGAUGCAAACCGCCUCCCGCUCAc -3' miRNA: 3'- -GACCCUACGUUUGGCGGAGGGCGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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