Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28406 | 5' | -55.5 | NC_005946.1 | + | 10253 | 0.66 | 0.889057 |
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Target: 5'- gGAGgGaaGCCUCCUCUACGcuggucaccgcCGGGGa -3' miRNA: 3'- gCUCaCgcUGGAGGAGAUGCa----------GUCCC- -5' |
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| 28406 | 5' | -55.5 | NC_005946.1 | + | 48026 | 0.66 | 0.882066 |
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Target: 5'- uGGGUggGCGGCCUCCUUgGCGaCAGu- -3' miRNA: 3'- gCUCA--CGCUGGAGGAGaUGCaGUCcc -5' |
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| 28406 | 5' | -55.5 | NC_005946.1 | + | 41417 | 0.66 | 0.871896 |
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Target: 5'- gCGGGgucccgGCgGACCgUCCUCUccgucccugccccCGUCAGGGu -3' miRNA: 3'- -GCUCa-----CG-CUGG-AGGAGAu------------GCAGUCCC- -5' |
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| 28406 | 5' | -55.5 | NC_005946.1 | + | 38365 | 0.67 | 0.843802 |
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Target: 5'- aGAGUGCGGgg-UCUCUACGaCGGGa -3' miRNA: 3'- gCUCACGCUggaGGAGAUGCaGUCCc -5' |
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| 28406 | 5' | -55.5 | NC_005946.1 | + | 72087 | 0.67 | 0.827068 |
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Target: 5'- aGAGgacaGCGugguccucaacGCCUCCUCgUugGaCAGGGg -3' miRNA: 3'- gCUCa---CGC-----------UGGAGGAG-AugCaGUCCC- -5' |
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| 28406 | 5' | -55.5 | NC_005946.1 | + | 66812 | 1.11 | 0.001912 |
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Target: 5'- uCGAGUGCGACCUCCUCUACGUCAGGGa -3' miRNA: 3'- -GCUCACGCUGGAGGAGAUGCAGUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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