Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28407 | 3' | -52.3 | NC_005946.1 | + | 16450 | 0.66 | 0.96334 |
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Target: 5'- gGUGcUGCCGUCUugCGACaucAAGGCCGc -3' miRNA: 3'- -UACuGCGGCAGA--GCUGcauUUUCGGC- -5' |
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| 28407 | 3' | -52.3 | NC_005946.1 | + | 21598 | 0.66 | 0.95163 |
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Target: 5'- -cGGcCGCCaUCUCcaacacCGUGAGAGCCGg -3' miRNA: 3'- uaCU-GCGGcAGAGcu----GCAUUUUCGGC- -5' |
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| 28407 | 3' | -52.3 | NC_005946.1 | + | 102358 | 0.67 | 0.94446 |
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Target: 5'- uUGACGUUgacauggcggugaggGUCUCGcCGUGGAGGuCCGu -3' miRNA: 3'- uACUGCGG---------------CAGAGCuGCAUUUUC-GGC- -5' |
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| 28407 | 3' | -52.3 | NC_005946.1 | + | 8244 | 0.69 | 0.87412 |
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Target: 5'- cAUGGCGCCGUCUggccacugccUGGCGUu-GGGUCu -3' miRNA: 3'- -UACUGCGGCAGA----------GCUGCAuuUUCGGc -5' |
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| 28407 | 3' | -52.3 | NC_005946.1 | + | 52933 | 0.7 | 0.824387 |
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Target: 5'- -cGAUGCUGUCcgcuccCGuCGUGAGAGCCu -3' miRNA: 3'- uaCUGCGGCAGa-----GCuGCAUUUUCGGc -5' |
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| 28407 | 3' | -52.3 | NC_005946.1 | + | 90796 | 0.7 | 0.806181 |
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Target: 5'- -aGGCGCaguaGUCgaCGACGUAguGAGGCCa -3' miRNA: 3'- uaCUGCGg---CAGa-GCUGCAU--UUUCGGc -5' |
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| 28407 | 3' | -52.3 | NC_005946.1 | + | 80351 | 0.72 | 0.706374 |
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Target: 5'- -aGGgGCCcUC-CGACGUGGAAGCCa -3' miRNA: 3'- uaCUgCGGcAGaGCUGCAUUUUCGGc -5' |
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| 28407 | 3' | -52.3 | NC_005946.1 | + | 67350 | 1.08 | 0.005057 |
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Target: 5'- cAUGACGCCGUCUCGACGUAAAAGCCGg -3' miRNA: 3'- -UACUGCGGCAGAGCUGCAUUUUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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