Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28409 | 3' | -57.6 | NC_005946.1 | + | 15628 | 0.66 | 0.742687 |
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Target: 5'- aUAGGAGacaucaccggaGGCCUGAGCUGagGA-GCg- -3' miRNA: 3'- gGUCCUC-----------CCGGACUCGACa-CUaCGac -5' |
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| 28409 | 3' | -57.6 | NC_005946.1 | + | 79500 | 0.69 | 0.556825 |
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Target: 5'- gCCuGGAGGGCCUGAcgGCcGagcUGGUGCc- -3' miRNA: 3'- -GGuCCUCCCGGACU--CGaC---ACUACGac -5' |
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| 28409 | 3' | -57.6 | NC_005946.1 | + | 58055 | 0.71 | 0.467459 |
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Target: 5'- cCCAcGGGGauauacGGCgaGGGCUGUGAccUGCUGg -3' miRNA: 3'- -GGU-CCUC------CCGgaCUCGACACU--ACGAC- -5' |
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| 28409 | 3' | -57.6 | NC_005946.1 | + | 28658 | 0.73 | 0.369285 |
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Target: 5'- gCCAGGGGGGCCgucagccugucGAGCUuccucGUGAUGUc- -3' miRNA: 3'- -GGUCCUCCCGGa----------CUCGA-----CACUACGac -5' |
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| 28409 | 3' | -57.6 | NC_005946.1 | + | 3076 | 0.75 | 0.279415 |
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Target: 5'- -gAGGGGGGCCUGuGGCUGgagagggGAcGCUGg -3' miRNA: 3'- ggUCCUCCCGGAC-UCGACa------CUaCGAC- -5' |
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| 28409 | 3' | -57.6 | NC_005946.1 | + | 69604 | 1.09 | 0.001177 |
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Target: 5'- uCCAGGAGGGCCUGAGCUGUGAUGCUGu -3' miRNA: 3'- -GGUCCUCCCGGACUCGACACUACGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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