Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28417 | 3' | -53.5 | NC_005946.1 | + | 74665 | 1.1 | 0.002901 |
Target: 5'- gUCUCCCGUGGACCUAAAGUCCGACAGg -3' miRNA: 3'- -AGAGGGCACCUGGAUUUCAGGCUGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 62647 | 0.7 | 0.749302 |
Target: 5'- -aUCCCGUcGGACCUcuuGGUgaCUGACGGg -3' miRNA: 3'- agAGGGCA-CCUGGAuu-UCA--GGCUGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 61859 | 0.7 | 0.755278 |
Target: 5'- cCUCCCGacgcccugcccagGGACCUGuaugugacGGGUCCGGCu- -3' miRNA: 3'- aGAGGGCa------------CCUGGAU--------UUCAGGCUGuc -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 36612 | 0.7 | 0.75825 |
Target: 5'- gUCUCCCaugccgaGUGGACCgccaccGGGUCCGAa-- -3' miRNA: 3'- -AGAGGG-------CACCUGGau----UUCAGGCUguc -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 104350 | 0.7 | 0.788284 |
Target: 5'- aUCUCCuCG-GGAgCUucgcAGUCUGACAGa -3' miRNA: 3'- -AGAGG-GCaCCUgGAuu--UCAGGCUGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 94623 | 0.69 | 0.824858 |
Target: 5'- cCUCCCGUGGAguaCCUGAcGUUCucgGGCAa -3' miRNA: 3'- aGAGGGCACCU---GGAUUuCAGG---CUGUc -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 43672 | 0.68 | 0.858432 |
Target: 5'- -gUCCCGUagagGGACCccAGGUCCGAg-- -3' miRNA: 3'- agAGGGCA----CCUGGauUUCAGGCUguc -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 10298 | 0.67 | 0.888507 |
Target: 5'- cUCUCaCCGgaucgGGagcGCCggggcauAGUCCGACGGg -3' miRNA: 3'- -AGAG-GGCa----CC---UGGauu----UCAGGCUGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 88075 | 0.67 | 0.895432 |
Target: 5'- cCUCCUGaaaGaGuACCUAGAGUCUGugGGa -3' miRNA: 3'- aGAGGGCa--C-C-UGGAUUUCAGGCugUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 12572 | 0.67 | 0.914717 |
Target: 5'- gUCUCCCGUGGAgaCCauaGGAGagaCGGCAa -3' miRNA: 3'- -AGAGGGCACCU--GGa--UUUCag-GCUGUc -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 60304 | 0.66 | 0.925749 |
Target: 5'- -gUCCuUGUaGACCcacacguUAAAGUCCGACGGg -3' miRNA: 3'- agAGG-GCAcCUGG-------AUUUCAGGCUGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 102096 | 0.66 | 0.926304 |
Target: 5'- --gUCCGUcaGGACCUGcaccgucucgGGGUCCGuCAGg -3' miRNA: 3'- agaGGGCA--CCUGGAU----------UUCAGGCuGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 78937 | 0.66 | 0.926304 |
Target: 5'- gUCUCCCGUcuGCCUGgcGUCgGGCu- -3' miRNA: 3'- -AGAGGGCAccUGGAUuuCAGgCUGuc -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 49030 | 0.66 | 0.931713 |
Target: 5'- cUCUgacaCCGUcacguacacGGGCCUAGAGUCCugguACAGg -3' miRNA: 3'- -AGAg---GGCA---------CCUGGAUUUCAGGc---UGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 17913 | 0.66 | 0.931713 |
Target: 5'- gCUCuuGUuguuGGGCCUcAGGUCaGACAGc -3' miRNA: 3'- aGAGggCA----CCUGGAuUUCAGgCUGUC- -5' |
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28417 | 3' | -53.5 | NC_005946.1 | + | 89774 | 0.66 | 0.943652 |
Target: 5'- cCUCUCGUagacaaagagggucaGGACCUGGGgaguGUCgGGCAGc -3' miRNA: 3'- aGAGGGCA---------------CCUGGAUUU----CAGgCUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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