Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28421 | 3' | -45.3 | NC_005946.1 | + | 68041 | 0.66 | 0.999888 |
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Target: 5'- ------cCAgCCUCACGCAccucuuGAGGAGg -3' miRNA: 3'- aaaauauGUgGGAGUGCGU------UUUCUCa -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 776 | 0.66 | 0.999888 |
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Target: 5'- ----cUACGCCCUCaACGguGAGGuuGUa -3' miRNA: 3'- aaaauAUGUGGGAG-UGCguUUUCu-CA- -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 8961 | 0.66 | 0.999851 |
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Target: 5'- ----cUugACCCUCAUGCccagcauAGAGUu -3' miRNA: 3'- aaaauAugUGGGAGUGCGuuu----UCUCA- -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 101446 | 0.67 | 0.999325 |
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Target: 5'- -----aGCaACCCUCAgagaGCAAGGGAGa -3' miRNA: 3'- aaaauaUG-UGGGAGUg---CGUUUUCUCa -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 37566 | 0.68 | 0.998699 |
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Target: 5'- gUUUUGUACAccuCCCUCACuauGGAGGGUu -3' miRNA: 3'- -AAAAUAUGU---GGGAGUGcguUUUCUCA- -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 91391 | 0.68 | 0.998403 |
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Target: 5'- aUUUUuUACACCCcCGCGCAGcGGAc- -3' miRNA: 3'- -AAAAuAUGUGGGaGUGCGUUuUCUca -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 16584 | 0.68 | 0.998052 |
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Target: 5'- --------cCCCUCACaGCAGAGGAGa -3' miRNA: 3'- aaaauauguGGGAGUG-CGUUUUCUCa -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 62212 | 0.69 | 0.995178 |
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Target: 5'- --cUGUuUACCCUCGCGCccGGGGGa -3' miRNA: 3'- aaaAUAuGUGGGAGUGCGuuUUCUCa -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 102447 | 0.75 | 0.905521 |
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Target: 5'- -cUUGUGCACCCUCugGUuAAAGucuGUg -3' miRNA: 3'- aaAAUAUGUGGGAGugCGuUUUCu--CA- -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 67312 | 0.77 | 0.830234 |
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Target: 5'- ---cGU-CACCCUCACGCAgacgguaGAAGAGUc -3' miRNA: 3'- aaaaUAuGUGGGAGUGCGU-------UUUCUCA- -5' |
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| 28421 | 3' | -45.3 | NC_005946.1 | + | 79150 | 1.05 | 0.030587 |
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Target: 5'- uUUUUAUACACCCUCACGCAAAAGAGUc -3' miRNA: 3'- -AAAAUAUGUGGGAGUGCGUUUUCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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