Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28424 | 3' | -59.7 | NC_005946.1 | + | 36275 | 0.66 | 0.621522 |
Target: 5'- uGugGuGGGCUccGGCGGCgguGGCGGGUc -3' miRNA: 3'- -CugC-CCUGAcuUCGUCGug-CCGCCCA- -5' |
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28424 | 3' | -59.7 | NC_005946.1 | + | 101231 | 0.67 | 0.590735 |
Target: 5'- aGGCaGGACcuaagGAAGCAGCACGaGCGa-- -3' miRNA: 3'- -CUGcCCUGa----CUUCGUCGUGC-CGCcca -5' |
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28424 | 3' | -59.7 | NC_005946.1 | + | 36421 | 0.71 | 0.342831 |
Target: 5'- --aGGGACgGAAGgGGCACGGaGGGa -3' miRNA: 3'- cugCCCUGaCUUCgUCGUGCCgCCCa -5' |
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28424 | 3' | -59.7 | NC_005946.1 | + | 40145 | 0.72 | 0.335177 |
Target: 5'- --aGGGGCUGGAGaGGCucucCGGCGGGc -3' miRNA: 3'- cugCCCUGACUUCgUCGu---GCCGCCCa -5' |
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28424 | 3' | -59.7 | NC_005946.1 | + | 81349 | 1.06 | 0.001319 |
Target: 5'- gGACGGGACUGAAGCAGCACGGCGGGUc -3' miRNA: 3'- -CUGCCCUGACUUCGUCGUGCCGCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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