Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28431 | 5' | -62.4 | NC_005946.1 | + | 18546 | 0.66 | 0.518113 |
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Target: 5'- cGGCCGaCUUGcuguAGGGGGCCUugUaCCAa -3' miRNA: 3'- -UCGGCaGGAC----UCCCUCGGGugAgGGU- -5' |
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| 28431 | 5' | -62.4 | NC_005946.1 | + | 14384 | 0.66 | 0.508478 |
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Target: 5'- uGCauaGUCCUGgccaGGGGAGCggCGCUCCuCAg -3' miRNA: 3'- uCGg--CAGGAC----UCCCUCGg-GUGAGG-GU- -5' |
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| 28431 | 5' | -62.4 | NC_005946.1 | + | 71776 | 0.66 | 0.498919 |
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Target: 5'- gGGCacgGUCCUGguGGGGAagguGUCuCACUCCCu -3' miRNA: 3'- -UCGg--CAGGAC--UCCCU----CGG-GUGAGGGu -5' |
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| 28431 | 5' | -62.4 | NC_005946.1 | + | 12627 | 0.68 | 0.383279 |
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Target: 5'- gGGCagaCGaCCUGAGGGAcaGCCUGC-CCCAg -3' miRNA: 3'- -UCG---GCaGGACUCCCU--CGGGUGaGGGU- -5' |
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| 28431 | 5' | -62.4 | NC_005946.1 | + | 95116 | 0.7 | 0.286869 |
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Target: 5'- aGGCgGUCCUGAGGa---UCGCUCCCAc -3' miRNA: 3'- -UCGgCAGGACUCCcucgGGUGAGGGU- -5' |
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| 28431 | 5' | -62.4 | NC_005946.1 | + | 27454 | 0.75 | 0.134266 |
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Target: 5'- gAGCC-UCCUGAGGG-GCCCGCcCUCGg -3' miRNA: 3'- -UCGGcAGGACUCCCuCGGGUGaGGGU- -5' |
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| 28431 | 5' | -62.4 | NC_005946.1 | + | 83126 | 1.08 | 0.000558 |
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Target: 5'- cAGCCGUCCUGAGGGAGCCCACUCCCAg -3' miRNA: 3'- -UCGGCAGGACUCCCUCGGGUGAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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