Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28433 | 5' | -49 | NC_005946.1 | + | 88121 | 0.66 | 0.99534 |
Target: 5'- -cCAAGAGacc---CUCUGCCGUGGa -3' miRNA: 3'- guGUUCUUgauaaaGAGACGGCGCCc -5' |
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28433 | 5' | -49 | NC_005946.1 | + | 19587 | 0.66 | 0.993639 |
Target: 5'- cCGCAGcguCUGUccuugcCUCUGCCGaCGGGa -3' miRNA: 3'- -GUGUUcuuGAUAaa----GAGACGGC-GCCC- -5' |
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28433 | 5' | -49 | NC_005946.1 | + | 56298 | 0.67 | 0.991472 |
Target: 5'- gACAAGGugggagACgucggcCUCUGCCGcCGGGu -3' miRNA: 3'- gUGUUCU------UGauaaa-GAGACGGC-GCCC- -5' |
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28433 | 5' | -49 | NC_005946.1 | + | 45078 | 0.67 | 0.991472 |
Target: 5'- gCACAGGugccuCUGccUCUCUGCCGCc-- -3' miRNA: 3'- -GUGUUCuu---GAUaaAGAGACGGCGccc -5' |
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28433 | 5' | -49 | NC_005946.1 | + | 84946 | 1.12 | 0.006274 |
Target: 5'- gCACAAGAACUAUUUCUCUGCCGCGGGa -3' miRNA: 3'- -GUGUUCUUGAUAAAGAGACGGCGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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