Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28435 | 5' | -51.8 | NC_005946.1 | + | 45003 | 0.66 | 0.967074 |
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Target: 5'- --uUCUUCAGAgAGuCGGggagGGCGGCa -3' miRNA: 3'- cacAGAAGUCU-UCuGUCgaa-CCGCCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 26980 | 0.67 | 0.937633 |
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Target: 5'- --cUCUUUAGccuGugGGcCUUGGUGGCg -3' miRNA: 3'- cacAGAAGUCuu-CugUC-GAACCGCCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 54380 | 0.67 | 0.932376 |
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Target: 5'- -aGUCUUCAGAcGACaaaaAGCU-GGacgaGGCu -3' miRNA: 3'- caCAGAAGUCUuCUG----UCGAaCCg---CCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 68971 | 0.67 | 0.926849 |
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Target: 5'- uUGUCcccagcuggUUCuuGAGGGCGGCUcUGGUGGUc -3' miRNA: 3'- cACAG---------AAGu-CUUCUGUCGA-ACCGCCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 84044 | 0.67 | 0.926282 |
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Target: 5'- -aGUCUUUAGAAacucuccaguccuGACcGacgUGGCGGCg -3' miRNA: 3'- caCAGAAGUCUU-------------CUGuCga-ACCGCCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 36286 | 0.68 | 0.888049 |
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Target: 5'- -gGUCUggaAGGuguGGugGGCUccGGCGGCg -3' miRNA: 3'- caCAGAag-UCU---UCugUCGAa-CCGCCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 36360 | 0.69 | 0.857043 |
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Target: 5'- -aGUCUcuuGAGGugGGCcgcGGCGGCg -3' miRNA: 3'- caCAGAaguCUUCugUCGaa-CCGCCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 32872 | 0.71 | 0.784562 |
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Target: 5'- cUGcCUgaacagCAGAGGGCAGCUUGGaCaGCu -3' miRNA: 3'- cACaGAa-----GUCUUCUGUCGAACC-GcCG- -5' |
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| 28435 | 5' | -51.8 | NC_005946.1 | + | 86428 | 1.13 | 0.002417 |
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Target: 5'- uGUGUCUUCAGAAGACAGCUUGGCGGCg -3' miRNA: 3'- -CACAGAAGUCUUCUGUCGAACCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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