Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28436 | 3' | -57 | NC_005946.1 | + | 26451 | 0.66 | 0.817458 |
Target: 5'- uGACGGgCUuGGucaagaccaccccgcAGAGGUCCCUGUccucgggaGCCg -3' miRNA: 3'- -CUGCCaGAcUC---------------UCUCCAGGGACG--------UGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 35243 | 0.66 | 0.813937 |
Target: 5'- gGACGG-CUGcGGGAuGUCCUcGUACCu -3' miRNA: 3'- -CUGCCaGACuCUCUcCAGGGaCGUGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 46316 | 0.67 | 0.763964 |
Target: 5'- aACGGacugcgacuucucCUGAGGGGGGUCCacaUGCcguACCg -3' miRNA: 3'- cUGCCa------------GACUCUCUCCAGGg--ACG---UGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 74745 | 0.68 | 0.688028 |
Target: 5'- uACGGgUUGGGuAGAGaGUCCCUGUugUu -3' miRNA: 3'- cUGCCaGACUC-UCUC-CAGGGACGugG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 10690 | 0.68 | 0.666382 |
Target: 5'- --gGGUCUGAcGgccagcagguccaGGAGGUCCCUccugacgaacgaGCACCc -3' miRNA: 3'- cugCCAGACU-C-------------UCUCCAGGGA------------CGUGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 86521 | 0.68 | 0.657061 |
Target: 5'- --gGGUUccaGGGAGAGGUUCCUGgACUc -3' miRNA: 3'- cugCCAGa--CUCUCUCCAGGGACgUGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 102346 | 0.69 | 0.646685 |
Target: 5'- uGGCGGUgaGGGucucgccguGGAGGUCCgUGUagACCa -3' miRNA: 3'- -CUGCCAgaCUC---------UCUCCAGGgACG--UGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 84009 | 0.71 | 0.533518 |
Target: 5'- cGGCGGgaagGAGGGuGGUgCCUGCuCCg -3' miRNA: 3'- -CUGCCaga-CUCUCuCCAgGGACGuGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 26559 | 0.71 | 0.513565 |
Target: 5'- -uUGGcCUGAGGGcAGGUCCCacCACCa -3' miRNA: 3'- cuGCCaGACUCUC-UCCAGGGacGUGG- -5' |
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28436 | 3' | -57 | NC_005946.1 | + | 86687 | 1.12 | 0.000943 |
Target: 5'- aGACGGUCUGAGAGAGGUCCCUGCACCa -3' miRNA: 3'- -CUGCCAGACUCUCUCCAGGGACGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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