Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28437 | 5' | -61.4 | NC_005946.1 | + | 27488 | 0.67 | 0.521717 |
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Target: 5'- uGGCCGCAgUCacggcguccaacUGCCucaugagGAGCCUCCUGaGGg -3' miRNA: 3'- -UCGGUGUgAG------------GCGG-------CUCGGAGGAC-CU- -5' |
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| 28437 | 5' | -61.4 | NC_005946.1 | + | 27960 | 0.67 | 0.503322 |
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Target: 5'- uGCUcgaaGCACUCUG-CGAGCCUCCUc-- -3' miRNA: 3'- uCGG----UGUGAGGCgGCUCGGAGGAccu -5' |
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| 28437 | 5' | -61.4 | NC_005946.1 | + | 65077 | 0.67 | 0.493754 |
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Target: 5'- cAGCCACuCUCCGCCu--CCgUCCUcGGAc -3' miRNA: 3'- -UCGGUGuGAGGCGGcucGG-AGGA-CCU- -5' |
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| 28437 | 5' | -61.4 | NC_005946.1 | + | 63456 | 0.67 | 0.474875 |
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Target: 5'- aGGCCACAguauUUuuGCCGGGgaUCCUGGc -3' miRNA: 3'- -UCGGUGU----GAggCGGCUCggAGGACCu -5' |
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| 28437 | 5' | -61.4 | NC_005946.1 | + | 9636 | 0.68 | 0.450885 |
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Target: 5'- gGGCCACagACaccccguccucgucaUCCGCCGccuccGCCUCCaGGAu -3' miRNA: 3'- -UCGGUG--UG---------------AGGCGGCu----CGGAGGaCCU- -5' |
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| 28437 | 5' | -61.4 | NC_005946.1 | + | 34669 | 0.7 | 0.331418 |
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Target: 5'- uGCC-CACgUCCGCCaGGUCgggCCUGGAc -3' miRNA: 3'- uCGGuGUG-AGGCGGcUCGGa--GGACCU- -5' |
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| 28437 | 5' | -61.4 | NC_005946.1 | + | 29764 | 0.7 | 0.331418 |
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Target: 5'- gGGCCACAgUuuGUaucccAGCCUCCUGGu -3' miRNA: 3'- -UCGGUGUgAggCGgc---UCGGAGGACCu -5' |
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| 28437 | 5' | -61.4 | NC_005946.1 | + | 86926 | 1.08 | 0.000624 |
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Target: 5'- gAGCCACACUCCGCCGAGCCUCCUGGAa -3' miRNA: 3'- -UCGGUGUGAGGCGGCUCGGAGGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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