Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28439 | 5' | -53.9 | NC_005946.1 | + | 72055 | 0.66 | 0.911562 |
Target: 5'- uGGACAGGGgccUCUUUUCCUGCGuUACc- -3' miRNA: 3'- -CCUGUUCUa--AGGAAGGGGCGC-GUGca -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 26808 | 0.66 | 0.911562 |
Target: 5'- uGGACAGGAccauggCC-UCCCUGUGCcUGUc -3' miRNA: 3'- -CCUGUUCUaa----GGaAGGGGCGCGuGCA- -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 13352 | 0.67 | 0.898725 |
Target: 5'- uGGACGGGg--CCUacauaUCCCUGCuGCugGg -3' miRNA: 3'- -CCUGUUCuaaGGA-----AGGGGCG-CGugCa -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 66593 | 0.67 | 0.880565 |
Target: 5'- cGGACGAGA-UCCUcgaguacgacaccucUCCCuuugagagguuCGUGCACGc -3' miRNA: 3'- -CCUGUUCUaAGGA---------------AGGG-----------GCGCGUGCa -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 82505 | 0.67 | 0.875399 |
Target: 5'- aGGCAGGAgaaacgUCCUUCCCUucaggcccaagacuGUGCACc- -3' miRNA: 3'- cCUGUUCUa-----AGGAAGGGG--------------CGCGUGca -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 82412 | 0.67 | 0.875399 |
Target: 5'- aGGCAGGAgaaacgUCCUUCCCUucaggcccgagacuGUGCACc- -3' miRNA: 3'- cCUGUUCUa-----AGGAAGGGG--------------CGCGUGca -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 62781 | 0.69 | 0.820527 |
Target: 5'- aGGcCAGGGcagCCUUCCaCGCGUugGUg -3' miRNA: 3'- -CCuGUUCUaa-GGAAGGgGCGCGugCA- -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 21096 | 0.69 | 0.820527 |
Target: 5'- cGGCGAGGUUC--UCCCCGC-CACa- -3' miRNA: 3'- cCUGUUCUAAGgaAGGGGCGcGUGca -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 19283 | 0.71 | 0.714066 |
Target: 5'- cGGGCAuGAUcuggugCCUUCCgCCGUGCAgGg -3' miRNA: 3'- -CCUGUuCUAa-----GGAAGG-GGCGCGUgCa -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 22943 | 0.71 | 0.702702 |
Target: 5'- -cACAGGGUUCCUggugggaUCCCUGCaaaaggccucGCACGUg -3' miRNA: 3'- ccUGUUCUAAGGA-------AGGGGCG----------CGUGCA- -5' |
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28439 | 5' | -53.9 | NC_005946.1 | + | 90888 | 1.09 | 0.003252 |
Target: 5'- gGGACAAGAUUCCUUCCCCGCGCACGUc -3' miRNA: 3'- -CCUGUUCUAAGGAAGGGGCGCGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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