Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28441 | 5' | -47.2 | NC_005946.1 | + | 92833 | 1.12 | 0.00949 |
Target: 5'- gCGAGAAUAGACAACACAAGACGCGACg -3' miRNA: 3'- -GCUCUUAUCUGUUGUGUUCUGCGCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 36426 | 0.7 | 0.977681 |
Target: 5'- gGAGGAgGGACGgaagggGCACGgagGGACGCGGa -3' miRNA: 3'- gCUCUUaUCUGU------UGUGU---UCUGCGCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 82341 | 0.7 | 0.984446 |
Target: 5'- aGAGGAUGGACGugucaGCGGGA-GCGAg -3' miRNA: 3'- gCUCUUAUCUGUug---UGUUCUgCGCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 79332 | 0.69 | 0.985772 |
Target: 5'- uCGAGGGgucagagucggcccUGGAUaaaGACGC-GGACGCGGCg -3' miRNA: 3'- -GCUCUU--------------AUCUG---UUGUGuUCUGCGCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 25780 | 0.69 | 0.988003 |
Target: 5'- gGAGGugGUGGACGACG-AGGACGaCGAg -3' miRNA: 3'- gCUCU--UAUCUGUUGUgUUCUGC-GCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 69045 | 0.68 | 0.994172 |
Target: 5'- uGAGGAUGGugGGCAUGAcucccuuuuuuGugGCGGg -3' miRNA: 3'- gCUCUUAUCugUUGUGUU-----------CugCGCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 21373 | 0.68 | 0.995024 |
Target: 5'- aGAGGgagGUGGAgAGCACcaGGGACGCcgugGACg -3' miRNA: 3'- gCUCU---UAUCUgUUGUG--UUCUGCG----CUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 61405 | 0.68 | 0.995772 |
Target: 5'- aGAGggUGGACcACGuCAAGAgGaUGGCc -3' miRNA: 3'- gCUCuuAUCUGuUGU-GUUCUgC-GCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 104283 | 0.68 | 0.995772 |
Target: 5'- uGAGAAgAGA-AACACAGauACGCGACg -3' miRNA: 3'- gCUCUUaUCUgUUGUGUUc-UGCGCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 82434 | 0.67 | 0.996993 |
Target: 5'- aGAGGAUGGACGugucaGCGGGAgGCa-- -3' miRNA: 3'- gCUCUUAUCUGUug---UGUUCUgCGcug -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 92024 | 0.67 | 0.997786 |
Target: 5'- aCGAGccUGGACAucuuuucggcguggACGCAcagcuuuacgugAGGCGUGACg -3' miRNA: 3'- -GCUCuuAUCUGU--------------UGUGU------------UCUGCGCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 95107 | 0.67 | 0.997906 |
Target: 5'- uGAGGAUcgcucccaccagGGACAACACccAGGCGgcaGACg -3' miRNA: 3'- gCUCUUA------------UCUGUUGUGu-UCUGCg--CUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 24500 | 0.67 | 0.997906 |
Target: 5'- cCGGGAaagauGUAGGC-GCACGccGugGCGGCc -3' miRNA: 3'- -GCUCU-----UAUCUGuUGUGUu-CugCGCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 34410 | 0.67 | 0.997906 |
Target: 5'- gGAGcacAUGGACAcCACGGGaACGUGAg -3' miRNA: 3'- gCUCu--UAUCUGUuGUGUUC-UGCGCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 44410 | 0.67 | 0.997906 |
Target: 5'- gGAGGAcAGGCAGCACAcuGCgguucugucuGCGGCc -3' miRNA: 3'- gCUCUUaUCUGUUGUGUucUG----------CGCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 58401 | 0.67 | 0.998268 |
Target: 5'- ----cGUAGACGGCGC-AGACGUGGu -3' miRNA: 3'- gcucuUAUCUGUUGUGuUCUGCGCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 88304 | 0.66 | 0.998575 |
Target: 5'- gCGAGGGgcacuuUAGGgAGCACGAugacGACGCGGu -3' miRNA: 3'- -GCUCUU------AUCUgUUGUGUU----CUGCGCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 1991 | 0.66 | 0.998575 |
Target: 5'- aCGGGAgcgugaccAUGGACGACGuCAAGAacacuauaaacUGUGACu -3' miRNA: 3'- -GCUCU--------UAUCUGUUGU-GUUCU-----------GCGCUG- -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 3878 | 0.66 | 0.998835 |
Target: 5'- aUGGGggUAaGCAACACGAGcauguCGUGGa -3' miRNA: 3'- -GCUCuuAUcUGUUGUGUUCu----GCGCUg -5' |
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28441 | 5' | -47.2 | NC_005946.1 | + | 71858 | 0.66 | 0.999053 |
Target: 5'- aGAGAA-AGGCGGC-CAAGAggccCGCGGg -3' miRNA: 3'- gCUCUUaUCUGUUGuGUUCU----GCGCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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