Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28442 | 3' | -58.7 | NC_005946.1 | + | 93878 | 0.66 | 0.73733 |
Target: 5'- cGUGGCCGCagcgguagaaGAGCC-CAGGGGacaGGCc -3' miRNA: 3'- -CGCUGGCGg---------CUCGGaGUCUCUca-CCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 20873 | 0.66 | 0.697625 |
Target: 5'- aGCaGGCCGCCGcgguGGCC-CuGAGGGcaaaGGCu -3' miRNA: 3'- -CG-CUGGCGGC----UCGGaGuCUCUCa---CCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 71976 | 0.66 | 0.697625 |
Target: 5'- gGUGGgUGCCuccccgacugGGGCCUgaggaaCAGAGAGUGGg -3' miRNA: 3'- -CGCUgGCGG----------CUCGGA------GUCUCUCACCg -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 77182 | 0.67 | 0.65704 |
Target: 5'- -gGGCCGCCaacauGGGUCUCcccaagcucauAGAGAG-GGCu -3' miRNA: 3'- cgCUGGCGG-----CUCGGAG-----------UCUCUCaCCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 56382 | 0.67 | 0.643755 |
Target: 5'- uGUGACgGCCGAGUC-CAG-GAGcccgucgugguacaUGGCc -3' miRNA: 3'- -CGCUGgCGGCUCGGaGUCuCUC--------------ACCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 103103 | 0.68 | 0.616133 |
Target: 5'- -gGACCGCCGGGaCCcaggGGAGAGaagGGUc -3' miRNA: 3'- cgCUGGCGGCUC-GGag--UCUCUCa--CCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 2478 | 0.68 | 0.575414 |
Target: 5'- gGUGAgCGCCGAGUgggaCUC-GAGGGgGGCc -3' miRNA: 3'- -CGCUgGCGGCUCG----GAGuCUCUCaCCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 66401 | 0.69 | 0.565318 |
Target: 5'- -aGACaGCCGuGCCUCAaucucugagcGAGAugGUGGCu -3' miRNA: 3'- cgCUGgCGGCuCGGAGU----------CUCU--CACCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 2286 | 0.69 | 0.565318 |
Target: 5'- cGCGGaccCCGCCGggGGCUggugCAGAaAGUGGUa -3' miRNA: 3'- -CGCU---GGCGGC--UCGGa---GUCUcUCACCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 47080 | 0.7 | 0.511757 |
Target: 5'- aCGGCCGCCGcaaaccccaucauGCCccuguggCGGuGAGUGGCc -3' miRNA: 3'- cGCUGGCGGCu------------CGGa------GUCuCUCACCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 72777 | 0.7 | 0.503026 |
Target: 5'- -gGGCCuGCCGGGCUUCuuuGAGGGcgcucccgccaggcUGGCg -3' miRNA: 3'- cgCUGG-CGGCUCGGAGu--CUCUC--------------ACCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 96512 | 0.7 | 0.486714 |
Target: 5'- uGCGAgaGCCugcagGAGCCUCugaaccGAGGGUGGg -3' miRNA: 3'- -CGCUggCGG-----CUCGGAGu-----CUCUCACCg -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 45505 | 0.7 | 0.466919 |
Target: 5'- -aGACUGCUG-GUCUCAGGGAGgcgucugaaaacaUGGCc -3' miRNA: 3'- cgCUGGCGGCuCGGAGUCUCUC-------------ACCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 103836 | 0.71 | 0.458563 |
Target: 5'- gGCGACCGUCGAgggcggcagaGUCUgggUGGAGGGUGGa -3' miRNA: 3'- -CGCUGGCGGCU----------CGGA---GUCUCUCACCg -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 98623 | 0.71 | 0.440285 |
Target: 5'- uGCGGCgGCCGugguGGCCUCAGcggacAGGGUGa- -3' miRNA: 3'- -CGCUGgCGGC----UCGGAGUC-----UCUCACcg -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 14016 | 0.72 | 0.363521 |
Target: 5'- aUGACCaaCGAGCCU-GGAGAGcUGGCg -3' miRNA: 3'- cGCUGGcgGCUCGGAgUCUCUC-ACCG- -5' |
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28442 | 3' | -58.7 | NC_005946.1 | + | 93937 | 1.03 | 0.003191 |
Target: 5'- cGCGACCGCC-AGCCUCAGAGAGUGGCg -3' miRNA: 3'- -CGCUGGCGGcUCGGAGUCUCUCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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