Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28446 | 3' | -53.5 | NC_005946.1 | + | 23929 | 0.66 | 0.946843 |
Target: 5'- cAGGUaGCCCUCGUugGGGaUGUAaaagACUu -3' miRNA: 3'- -UCCG-CGGGAGCAugCUUaGCAUg---UGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 32425 | 0.66 | 0.942247 |
Target: 5'- uGGGa-CCCUUGUugGuGUCGUaGCACa -3' miRNA: 3'- -UCCgcGGGAGCAugCuUAGCA-UGUGg -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 81820 | 0.66 | 0.942247 |
Target: 5'- cGGCGaCCCUCagGgGAcUCG-ACGCCa -3' miRNA: 3'- uCCGC-GGGAGcaUgCUuAGCaUGUGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 79233 | 0.66 | 0.9323 |
Target: 5'- cGGCGgCCUC-UGCGAcuAUgG-ACGCCa -3' miRNA: 3'- uCCGCgGGAGcAUGCU--UAgCaUGUGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 66333 | 0.66 | 0.926947 |
Target: 5'- aGGGaGaCCC-CGUACGucucuucggCGUGCGCCg -3' miRNA: 3'- -UCCgC-GGGaGCAUGCuua------GCAUGUGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 7342 | 0.67 | 0.915479 |
Target: 5'- cGGCaCCuCUCaGUGCGA---GUGCACCg -3' miRNA: 3'- uCCGcGG-GAG-CAUGCUuagCAUGUGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 68594 | 0.67 | 0.909366 |
Target: 5'- uGGGCGCCUauguagucuUCGU-CGGGcUCGUcagACACUg -3' miRNA: 3'- -UCCGCGGG---------AGCAuGCUU-AGCA---UGUGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 42992 | 0.67 | 0.909366 |
Target: 5'- uGGCcagCCUCuUGCGAccGUCGUugACCg -3' miRNA: 3'- uCCGcg-GGAGcAUGCU--UAGCAugUGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 48022 | 0.68 | 0.888837 |
Target: 5'- uGGGCGgCCUCcuugGCGAcagucuuGUCGUACAa- -3' miRNA: 3'- -UCCGCgGGAGca--UGCU-------UAGCAUGUgg -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 79003 | 0.68 | 0.859756 |
Target: 5'- cGGCGgCCaCGgagGCGg--CGUACGCCa -3' miRNA: 3'- uCCGCgGGaGCa--UGCuuaGCAUGUGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 27407 | 0.7 | 0.79955 |
Target: 5'- cGGUGCCCUgGUugGAuUC-UAC-CCa -3' miRNA: 3'- uCCGCGGGAgCAugCUuAGcAUGuGG- -5' |
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28446 | 3' | -53.5 | NC_005946.1 | + | 99080 | 1.13 | 0.002075 |
Target: 5'- aAGGCGCCCUCGUACGAAUCGUACACCa -3' miRNA: 3'- -UCCGCGGGAGCAUGCUUAGCAUGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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