Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28446 | 5' | -59.3 | NC_005946.1 | + | 36271 | 0.66 | 0.701282 |
Target: 5'- gUGGGCUcCGgcggcgguggcggGUCUGGgGGGAGcGGCCu -3' miRNA: 3'- -ACUCGGaGU-------------UAGGCCgCCCUCcUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 14584 | 0.66 | 0.692205 |
Target: 5'- aGAGCCUgGcgUCGGaCGuugccaugaGGAGG-GCCg -3' miRNA: 3'- aCUCGGAgUuaGGCC-GC---------CCUCCuCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 3480 | 0.66 | 0.670879 |
Target: 5'- --cGCCUCGgacGUCCuccuaaacaccggGGCGGGuguAGGGGUCu -3' miRNA: 3'- acuCGGAGU---UAGG-------------CCGCCC---UCCUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 56484 | 0.66 | 0.661691 |
Target: 5'- cGGGaCCUCAaaagacaucccGUCCuGCGGGGcguggaccucuGGAGUCg -3' miRNA: 3'- aCUC-GGAGU-----------UAGGcCGCCCU-----------CCUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 77247 | 0.66 | 0.661691 |
Target: 5'- cGGGCC--AGUCU-GCGGGcuGGAGCCc -3' miRNA: 3'- aCUCGGagUUAGGcCGCCCu-CCUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 55659 | 0.66 | 0.65146 |
Target: 5'- aUGGGCaaCGGgaCGGUGGGguugAGGAGCCc -3' miRNA: 3'- -ACUCGgaGUUagGCCGCCC----UCCUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 80585 | 0.67 | 0.645314 |
Target: 5'- cUGAGCCUCAgaugucgcuaagaacGUCCGGCcaucccGGGGCa -3' miRNA: 3'- -ACUCGGAGU---------------UAGGCCGcccu--CCUCGg -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 24723 | 0.67 | 0.630961 |
Target: 5'- cGGGUUUUggugGAUCCGGUuuGGGGGGguugGGCCc -3' miRNA: 3'- aCUCGGAG----UUAGGCCG--CCCUCC----UCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 39362 | 0.67 | 0.610465 |
Target: 5'- cUGcGUCUUcuGAUCUGGCGGGGgaucuuacuGGAGUCu -3' miRNA: 3'- -ACuCGGAG--UUAGGCCGCCCU---------CCUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 86208 | 0.68 | 0.57986 |
Target: 5'- aGAGCCUCcAUCC--CGGGuaGAGCCg -3' miRNA: 3'- aCUCGGAGuUAGGccGCCCucCUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 27488 | 0.68 | 0.573774 |
Target: 5'- -uGGCCgCAGUCaCGGCGuccaacugccucauGAGGAGCCu -3' miRNA: 3'- acUCGGaGUUAG-GCCGCc-------------CUCCUCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 23632 | 0.68 | 0.539608 |
Target: 5'- aGAGUCgCAGgccccugccCCGGCGGuGAGGGGUa -3' miRNA: 3'- aCUCGGaGUUa--------GGCCGCC-CUCCUCGg -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 36450 | 0.68 | 0.539608 |
Target: 5'- aGGGCC-CAAgCCGGUccgugacaGGGAGGAGg- -3' miRNA: 3'- aCUCGGaGUUaGGCCG--------CCCUCCUCgg -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 63711 | 0.69 | 0.490725 |
Target: 5'- -uGGCCUCcAUCaCGGCGcacauGGAGGGGUa -3' miRNA: 3'- acUCGGAGuUAG-GCCGC-----CCUCCUCGg -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 92544 | 0.69 | 0.471747 |
Target: 5'- cGAGCaugUCGuGUUCGGCgGGGAGGAucuGCCc -3' miRNA: 3'- aCUCGg--AGU-UAGGCCG-CCCUCCU---CGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 103015 | 0.7 | 0.462398 |
Target: 5'- cGGGUCUCc--CCGGagcaaaGGGAGG-GCCg -3' miRNA: 3'- aCUCGGAGuuaGGCCg-----CCCUCCuCGG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 97201 | 0.7 | 0.443089 |
Target: 5'- gGGGUUUCAcuAUCCucugauuGGUGGGAGGAGgCu -3' miRNA: 3'- aCUCGGAGU--UAGG-------CCGCCCUCCUCgG- -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 68715 | 0.71 | 0.408477 |
Target: 5'- aGuGCCUgGAUCCacGCGGGuGGAGUa -3' miRNA: 3'- aCuCGGAgUUAGGc-CGCCCuCCUCGg -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 61634 | 0.71 | 0.399877 |
Target: 5'- gGGGCCUUu-UUCGGCaGGGAGGAcaGCa -3' miRNA: 3'- aCUCGGAGuuAGGCCG-CCCUCCU--CGg -5' |
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28446 | 5' | -59.3 | NC_005946.1 | + | 86954 | 0.72 | 0.358658 |
Target: 5'- gGAGCguaCUCGGgaacCUGGCGuGGuGGAGCCa -3' miRNA: 3'- aCUCG---GAGUUa---GGCCGC-CCuCCUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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