miRNA: 3'- acuCGGAGU---UAGG-------------CCGCCC---UCCUCGG- -5'
miRNA: 3'- aCUCGGAgUuaGGCC-GC---------CCUCCuCGG- -5'
miRNA: 3'- -ACUCGGaGU-------------UAGGCCgCCCUCcUCGG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28446 | 5' | -59.3 | NC_005946.1 | + | 3480 | 0.66 | 0.670879 |
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Target: 5'- --cGCCUCGgacGUCCuccuaaacaccggGGCGGGuguAGGGGUCu -3' miRNA: 3'- acuCGGAGU---UAGG-------------CCGCCC---UCCUCGG- -5' |
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| 28446 | 5' | -59.3 | NC_005946.1 | + | 14584 | 0.66 | 0.692205 |
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Target: 5'- aGAGCCUgGcgUCGGaCGuugccaugaGGAGG-GCCg -3' miRNA: 3'- aCUCGGAgUuaGGCC-GC---------CCUCCuCGG- -5' |
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| 28446 | 5' | -59.3 | NC_005946.1 | + | 36271 | 0.66 | 0.701282 |
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Target: 5'- gUGGGCUcCGgcggcgguggcggGUCUGGgGGGAGcGGCCu -3' miRNA: 3'- -ACUCGGaGU-------------UAGGCCgCCCUCcUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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