Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28447 | 3' | -55 | NC_005946.1 | + | 74513 | 0.66 | 0.844607 |
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Target: 5'- uGACgGCG-GCGGcCUCCuccUCGGCCUCg -3' miRNA: 3'- -UUGgCGCgUGUUaGAGGu--AGUCGGAG- -5' |
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| 28447 | 3' | -55 | NC_005946.1 | + | 91704 | 0.66 | 0.836032 |
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Target: 5'- uGCC-CGCACucugGGUCccCCAUgAGCCUCa -3' miRNA: 3'- uUGGcGCGUG----UUAGa-GGUAgUCGGAG- -5' |
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| 28447 | 3' | -55 | NC_005946.1 | + | 9578 | 0.66 | 0.815545 |
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Target: 5'- cACCGCGUcggccaccauccucACcGUCUCC-UCgGGCCUCu -3' miRNA: 3'- uUGGCGCG--------------UGuUAGAGGuAG-UCGGAG- -5' |
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| 28447 | 3' | -55 | NC_005946.1 | + | 44687 | 0.67 | 0.809111 |
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Target: 5'- uGACUGCuGCACucuuuauaccUCUgaauauuauccCCGUCAGCCUCa -3' miRNA: 3'- -UUGGCG-CGUGuu--------AGA-----------GGUAGUCGGAG- -5' |
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| 28447 | 3' | -55 | NC_005946.1 | + | 10453 | 0.67 | 0.809111 |
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Target: 5'- gGACUggGCGCACAGgacacCCA-CAGCCUCc -3' miRNA: 3'- -UUGG--CGCGUGUUaga--GGUaGUCGGAG- -5' |
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| 28447 | 3' | -55 | NC_005946.1 | + | 88607 | 0.67 | 0.79977 |
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Target: 5'- uGGCCGUGgACGAUgaguaCUCCAUagaCGGCCUg -3' miRNA: 3'- -UUGGCGCgUGUUA-----GAGGUA---GUCGGAg -5' |
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| 28447 | 3' | -55 | NC_005946.1 | + | 68911 | 0.69 | 0.688232 |
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Target: 5'- gGACCGUGUugAgaGUgUCCAccacCAGCCUCc -3' miRNA: 3'- -UUGGCGCGugU--UAgAGGUa---GUCGGAG- -5' |
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| 28447 | 3' | -55 | NC_005946.1 | + | 99830 | 1.06 | 0.002798 |
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Target: 5'- cAACCGCGCACAAUCUCCAUCAGCCUCc -3' miRNA: 3'- -UUGGCGCGUGUUAGAGGUAGUCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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