Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28448 | 3' | -56.3 | NC_005946.1 | + | 23353 | 0.66 | 0.775396 |
Target: 5'- -cAGCCACCCUCccuugucCGUGGCGCAGa-- -3' miRNA: 3'- acUUGGUGGGAGu------GCGUCGUGUCgag -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 54248 | 0.67 | 0.75974 |
Target: 5'- -aGGCCGCUCUaguccaACGCcucauagaggcagacGGCAUAGCUCa -3' miRNA: 3'- acUUGGUGGGAg-----UGCG---------------UCGUGUCGAG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 72988 | 0.67 | 0.745785 |
Target: 5'- gUGAGCCA-CCUCAgGC-GCACAuGCa- -3' miRNA: 3'- -ACUUGGUgGGAGUgCGuCGUGU-CGag -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 8968 | 0.68 | 0.662863 |
Target: 5'- gGAGCCucuugACCCUCAUGCccAGCAUAGa-- -3' miRNA: 3'- aCUUGG-----UGGGAGUGCG--UCGUGUCgag -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 14800 | 0.68 | 0.652274 |
Target: 5'- aGAGCCagggcACCCUCACccuugaagGCgAGCACGGUUa -3' miRNA: 3'- aCUUGG-----UGGGAGUG--------CG-UCGUGUCGAg -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 34383 | 0.68 | 0.652274 |
Target: 5'- aGGGCC-CCCUCuCGCc-CACGGUUCg -3' miRNA: 3'- aCUUGGuGGGAGuGCGucGUGUCGAG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 63293 | 0.69 | 0.598195 |
Target: 5'- gUGGACCACCCcCACagagugaGCGGCgGCGGC-Cg -3' miRNA: 3'- -ACUUGGUGGGaGUG-------CGUCG-UGUCGaG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 79419 | 0.7 | 0.546869 |
Target: 5'- -cGACCGCCCUgGCGCuGGCGuCGGC-Ca -3' miRNA: 3'- acUUGGUGGGAgUGCG-UCGU-GUCGaG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 27944 | 0.71 | 0.496024 |
Target: 5'- cGAGCC-UCCUCGCGUGGUccuGCAcGCUCu -3' miRNA: 3'- aCUUGGuGGGAGUGCGUCG---UGU-CGAG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 67320 | 0.71 | 0.476282 |
Target: 5'- cUGAAgcccgUCACCCUCACGCAG-ACGGUa- -3' miRNA: 3'- -ACUU-----GGUGGGAGUGCGUCgUGUCGag -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 11142 | 0.72 | 0.466558 |
Target: 5'- gUGAGCCGgguacagguuguCCCcCACGCAGCuaaacauUAGCUCg -3' miRNA: 3'- -ACUUGGU------------GGGaGUGCGUCGu------GUCGAG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 15523 | 0.72 | 0.456938 |
Target: 5'- aGGGCCGCCacaaUCAC-CAGCAUAGC-Ca -3' miRNA: 3'- aCUUGGUGGg---AGUGcGUCGUGUCGaG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 4332 | 0.73 | 0.401588 |
Target: 5'- aGGGCCACCC-CGCGUGGUAguuUAGUUCg -3' miRNA: 3'- aCUUGGUGGGaGUGCGUCGU---GUCGAG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 11328 | 0.8 | 0.145167 |
Target: 5'- --cGCCACCCUCGCGCcggcuccGGCACAguGCUCa -3' miRNA: 3'- acuUGGUGGGAGUGCG-------UCGUGU--CGAG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 97492 | 0.8 | 0.137984 |
Target: 5'- gUGAACCACCC-CACGgGGUAguGCUCc -3' miRNA: 3'- -ACUUGGUGGGaGUGCgUCGUguCGAG- -5' |
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28448 | 3' | -56.3 | NC_005946.1 | + | 102234 | 1.1 | 0.001234 |
Target: 5'- cUGAACCACCCUCACGCAGCACAGCUCg -3' miRNA: 3'- -ACUUGGUGGGAGUGCGUCGUGUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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