Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28448 | 5' | -61.8 | NC_005946.1 | + | 57006 | 0.66 | 0.610719 |
Target: 5'- -cGGGGUGUCUG-GaAGGuccaGGAGCCUGg -3' miRNA: 3'- gcCCCCGCAGACgCaUCC----CCUUGGGC- -5' |
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28448 | 5' | -61.8 | NC_005946.1 | + | 13656 | 0.66 | 0.580895 |
Target: 5'- uGGGGauCGgCUGCGgAGGccaGGAACCCGa -3' miRNA: 3'- gCCCCc-GCaGACGCaUCC---CCUUGGGC- -5' |
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28448 | 5' | -61.8 | NC_005946.1 | + | 12380 | 0.66 | 0.579905 |
Target: 5'- cCGGGGagcaaacgccaagGUGUaCgacgGcCGUAGGuGGAACCCGg -3' miRNA: 3'- -GCCCC-------------CGCA-Ga---C-GCAUCC-CCUUGGGC- -5' |
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28448 | 5' | -61.8 | NC_005946.1 | + | 34440 | 0.68 | 0.466219 |
Target: 5'- gGGGacGGCuauGUCgGCGUAGGuGGGACCgGa -3' miRNA: 3'- gCCC--CCG---CAGaCGCAUCC-CCUUGGgC- -5' |
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28448 | 5' | -61.8 | NC_005946.1 | + | 3456 | 0.69 | 0.388378 |
Target: 5'- -cGGGGCGg--GUGUAGGGGucuACgCCGg -3' miRNA: 3'- gcCCCCGCagaCGCAUCCCCu--UG-GGC- -5' |
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28448 | 5' | -61.8 | NC_005946.1 | + | 60248 | 0.7 | 0.346709 |
Target: 5'- gGGGGGCaGUCUGCugcuGGGuccacuuuuucggaGAGCCCGu -3' miRNA: 3'- gCCCCCG-CAGACGcau-CCC--------------CUUGGGC- -5' |
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28448 | 5' | -61.8 | NC_005946.1 | + | 102200 | 1.08 | 0.000726 |
Target: 5'- uCGGGGGCGUCUGCGUAGGGGAACCCGa -3' miRNA: 3'- -GCCCCCGCAGACGCAUCCCCUUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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