Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28449 | 3' | -63.1 | NC_005946.1 | + | 101503 | 0.67 | 0.469388 |
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Target: 5'- aACUGUUUggacgugagaagaagGGCaAGGACCGUccccgggaagaCGGGACUCa -3' miRNA: 3'- -UGACAGG---------------CCG-UCCUGGCG-----------GCCCUGGG- -5' |
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| 28449 | 3' | -63.1 | NC_005946.1 | + | 8934 | 0.67 | 0.473934 |
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Target: 5'- uCUGUgCaGGguGGGCUGCCuguugagggccacGGGAUCCc -3' miRNA: 3'- uGACAgG-CCguCCUGGCGG-------------CCCUGGG- -5' |
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| 28449 | 3' | -63.1 | NC_005946.1 | + | 41435 | 0.66 | 0.493265 |
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Target: 5'- cACggGUCCGuG-AGGuCCGCgGGGuCCCg -3' miRNA: 3'- -UGa-CAGGC-CgUCCuGGCGgCCCuGGG- -5' |
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| 28449 | 3' | -63.1 | NC_005946.1 | + | 40222 | 0.66 | 0.502596 |
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Target: 5'- gACUGgguuuggaaGGCAGGACCaUCuGGGCCCu -3' miRNA: 3'- -UGACagg------CCGUCCUGGcGGcCCUGGG- -5' |
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| 28449 | 3' | -63.1 | NC_005946.1 | + | 75827 | 0.66 | 0.519583 |
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Target: 5'- -aUGgcgUCGGCgAGGACCacguccuGCCGGGcuauggcGCCCg -3' miRNA: 3'- ugACa--GGCCG-UCCUGG-------CGGCCC-------UGGG- -5' |
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| 28449 | 3' | -63.1 | NC_005946.1 | + | 10293 | 0.66 | 0.531032 |
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Target: 5'- ----aCCGGauCGGGAgCGCCGGGGCa- -3' miRNA: 3'- ugacaGGCC--GUCCUgGCGGCCCUGgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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